Silvia Luiza Saggiomo1, Christine Zelenka2, Jamie Seymour3. 1. Australian Institute of Tropical Health and Medicine, James Cook University, McGregor Road, Cairns Campus, Australia. Electronic address: silvia.saggiomo@my.jcu.edu.au. 2. Australian Institute of Tropical Health and Medicine, James Cook University, McGregor Road, Cairns Campus, Australia. Electronic address: christine.zelenka@my.jcu.edu.au. 3. Australian Institute of Tropical Health and Medicine, James Cook University, McGregor Road, Cairns Campus, Australia. Electronic address: jamie.seymour@jcu.edu.au.
Abstract
BACKGROUND: The potential costs of venom production may be significant to many marine venomous taxa. In general, the parameters that influence the rate of venom production are poorly understood, but seem to be related to feeding frequency. METHODS: This study examines the effects of starvation on venom profile and venom yield on the estuarine stonefish (Synanceia horrida). In total, the venom of eight stonefishes was tested under two feeding regimes. Over a four week period, one of the two groups underwent an episode of suspended feeding, while the other was fed on a daily basis. The effect of time on venom replacement was determined by a paired T-test. ANOVA was performed to analyze differences in venom weight between fed and unfed treatments. RESULTS: Nutritional suspension was found to have a significant effect on the quantity of venom produced. SDS-PAGE gel and FPLC revealed that the components of the venom collected from both groups were similar, indicating that four weeks is an adequate time to regenerate key venom components but not replenish initial venom quantities. CONCLUSIONS: Venom production was found to be affected by starvation.
BACKGROUND: The potential costs of venom production may be significant to many marine venomous taxa. In general, the parameters that influence the rate of venom production are poorly understood, but seem to be related to feeding frequency. METHODS: This study examines the effects of starvation on venom profile and venom yield on the estuarine stonefish (Synanceia horrida). In total, the venom of eight stonefishes was tested under two feeding regimes. Over a four week period, one of the two groups underwent an episode of suspended feeding, while the other was fed on a daily basis. The effect of time on venom replacement was determined by a paired T-test. ANOVA was performed to analyze differences in venom weight between fed and unfed treatments. RESULTS: Nutritional suspension was found to have a significant effect on the quantity of venom produced. SDS-PAGE gel and FPLC revealed that the components of the venom collected from both groups were similar, indicating that four weeks is an adequate time to regenerate key venom components but not replenish initial venom quantities. CONCLUSIONS: Venom production was found to be affected by starvation.
Authors: Bjoern M von Reumont; Gregor Anderluh; Agostinho Antunes; Naira Ayvazyan; Dimitris Beis; Figen Caliskan; Ana Crnković; Maik Damm; Sebastien Dutertre; Lars Ellgaard; Goran Gajski; Hannah German; Beata Halassy; Benjamin-Florian Hempel; Tim Hucho; Nasit Igci; Maria P Ikonomopoulou; Izhar Karbat; Maria I Klapa; Ivan Koludarov; Jeroen Kool; Tim Lüddecke; Riadh Ben Mansour; Maria Vittoria Modica; Yehu Moran; Ayse Nalbantsoy; María Eugenia Pachón Ibáñez; Alexios Panagiotopoulos; Eitan Reuveny; Javier Sánchez Céspedes; Andy Sombke; Joachim M Surm; Eivind A B Undheim; Aida Verdes; Giulia Zancolli Journal: Gigascience Date: 2022-05-18 Impact factor: 7.658
Authors: Tim Lüddecke; Björn M von Reumont; Frank Förster; André Billion; Thomas Timm; Günter Lochnit; Andreas Vilcinskas; Sarah Lemke Journal: Biomolecules Date: 2020-06-30
Authors: Silvia L Saggiomo; Cadhla Firth; David T Wilson; Jamie Seymour; John J Miles; Yide Wong Journal: Mar Drugs Date: 2021-05-24 Impact factor: 5.118