Flávia Paiva Prudente de Moraes1, Silvia Vanessa Lourenço2, Renata Carolina Fraga Ianez1, Elen Alves de Sousa1, Marlon Messias da Conceição Silva1, Aline Santos Damascena3, Luiz Paulo Kowalski4, Fernando Augusto Soares5, Cláudia Malheiros Coutinho-Camillo6. 1. International Research Center, AC Camargo Cancer Center, São Paulo, SP, Brazil; Department of Pathology, AC Camargo Cancer Center, São Paulo, SP, Brazil. 2. Department of General Pathology, Dental School, University of São Paulo, São Paulo, SP, Brazil. 3. International Research Center, AC Camargo Cancer Center, São Paulo, SP, Brazil. 4. Department of Head and Neck Surgery and Otorhinolaryngology, AC Camargo Cancer Center, São Paulo, SP, Brazil. 5. Department of Pathology, AC Camargo Cancer Center, São Paulo, SP, Brazil; Department of General Pathology, Dental School, University of São Paulo, São Paulo, SP, Brazil. 6. International Research Center, AC Camargo Cancer Center, São Paulo, SP, Brazil; Department of Pathology, AC Camargo Cancer Center, São Paulo, SP, Brazil. Electronic address: ccamilo@cipe.accamargo.org.br.
Abstract
OBJECTIVES: The aim of this study was to analyze the expression of CD24, CD44, CD133, ALDH1, CD29 (integrin-β1), and Ki-67 in squamous cell carcinoma of the oral cavity and oropharynx. STUDY DESIGN: Fifty-two tumors and 21 metastatic lymph nodes were evaluated by using immunohistochemistry. RESULTS: Seven of 52 cases (13.5%) showed positive cytoplasmic staining of aldehyde dehydrogenase 1; integrin-β1 was expressed in 45 of 50 cases (90%); 30 of 52 cases (57.7%) had positive membranous staining of CD44; CD24 was expressed in 44 of 50 cases (88%); and three of 52 cases (5.8%) stained positively for membranous CD133. Median proliferation rate, measured by Ki-67, was 37.1% for tumors. Five-year cancer-specific survival rates for the CD44-negative and CD44-positive groups were 74% and 38%, respectively, although this difference did not reach statistical significance (P = .052). CONCLUSIONS: Our study demonstrated the expression of putative stem cell markers in squamous cell carcinoma of the oral cavity and oropharynx, with participation of CD44-positive cells in association with poor survival outcome.
OBJECTIVES: The aim of this study was to analyze the expression of CD24, CD44, CD133, ALDH1, CD29 (integrin-β1), and Ki-67 in squamous cell carcinoma of the oral cavity and oropharynx. STUDY DESIGN: Fifty-two tumors and 21 metastatic lymph nodes were evaluated by using immunohistochemistry. RESULTS: Seven of 52 cases (13.5%) showed positive cytoplasmic staining of aldehyde dehydrogenase 1; integrin-β1 was expressed in 45 of 50 cases (90%); 30 of 52 cases (57.7%) had positive membranous staining of CD44; CD24 was expressed in 44 of 50 cases (88%); and three of 52 cases (5.8%) stained positively for membranous CD133. Median proliferation rate, measured by Ki-67, was 37.1% for tumors. Five-year cancer-specific survival rates for the CD44-negative and CD44-positive groups were 74% and 38%, respectively, although this difference did not reach statistical significance (P = .052). CONCLUSIONS: Our study demonstrated the expression of putative stem cell markers in squamous cell carcinoma of the oral cavity and oropharynx, with participation of CD44-positive cells in association with poor survival outcome.
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