Natalia Moreno-Castellanos1,2,3, Amaia Rodríguez3,4, Yoana Rabanal-Ruiz1,2,3, Alejandro Fernández-Vega1,2,3, José López-Miranda3,5, Rafael Vázquez-Martínez1,2,3, Gema Frühbeck6,7,8, María M Malagón9,10,11. 1. Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)/University of Córdoba/Reina Sofia University Hospital, Edificio IMIBIC, Avda. Menéndez Pidal s/n, 14004, Córdoba, Spain. 2. Department of Cell Biology, Physiology and Immunology, University of Cordoba, Córdoba, Spain. 3. CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Spain. 4. Metabolic Research Laboratory, Clínica Universidad de Navarra, IdiSNA, Pamplona, Spain. 5. Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Córdoba, Córdoba, Spain. 6. CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Spain, . gfruhbeck@unav.es. 7. Metabolic Research Laboratory, Clínica Universidad de Navarra, IdiSNA, Pamplona, Spain. gfruhbeck@unav.es. 8. Department of Endocrinology & Nutrition, Clínica Universidad de Navarra, Avda. Pío XII 36, 31008, Pamplona, Spain. gfruhbeck@unav.es. 9. Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)/University of Córdoba/Reina Sofia University Hospital, Edificio IMIBIC, Avda. Menéndez Pidal s/n, 14004, Córdoba, Spain. bc1mapom@uco.es. 10. Department of Cell Biology, Physiology and Immunology, University of Cordoba, Córdoba, Spain. bc1mapom@uco.es. 11. CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Spain, . bc1mapom@uco.es.
Abstract
AIMS/HYPOTHESIS: Septins are newly identified members of the cytoskeleton that have been proposed as biomarkers of a number of diseases. However, septins have not been characterised in adipose tissue and their relationship with obesity and insulin resistance remains unknown. Herein, we characterised a member of this family, septin 11 (SEPT11), in human adipose tissue and analysed its potential involvement in the regulation of adipocyte metabolism. METHODS: Gene and protein expression levels of SEPT11 were analysed in human adipose tissue. SEPT11 distribution was evaluated by immunocytochemistry, electron microscopy and subcellular fractionation techniques. Glutathione S-transferase (GST) pull-down, immunoprecipitation and yeast two-hybrid screening were used to identify the SEPT11 interactome. Gene silencing was used to assess the role of SEPT11 in the regulation of insulin signalling and lipid metabolism in adipocytes. RESULTS: We demonstrate the expression of SEPT11 in human adipocytes and its upregulation in obese individuals, with SEPT11 mRNA content positively correlating with variables of insulin resistance in subcutaneous adipose tissue. SEPT11 content was regulated by lipogenic, lipolytic and proinflammatory stimuli in human adipocytes. SEPT11 associated with caveolae in mature adipocytes and interacted with both caveolin-1 and the intracellular fatty acid chaperone, fatty acid binding protein 5 (FABP5). Lipid loading of adipocytes caused the association of the three proteins with the surface of lipid droplets. SEPT11 silencing impaired insulin signalling and insulin-induced lipid accumulation in adipocytes. CONCLUSIONS/ INTERPRETATION: Our findings support a role for SEPT11 in lipid traffic and metabolism in adipocytes and open new avenues for research on the control of lipid storage in obesity and insulin resistance.
AIMS/HYPOTHESIS: Septins are newly identified members of the cytoskeleton that have been proposed as biomarkers of a number of diseases. However, septins have not been characterised in adipose tissue and their relationship with obesity and insulin resistance remains unknown. Herein, we characterised a member of this family, septin 11 (SEPT11), in human adipose tissue and analysed its potential involvement in the regulation of adipocyte metabolism. METHODS: Gene and protein expression levels of SEPT11 were analysed in human adipose tissue. SEPT11 distribution was evaluated by immunocytochemistry, electron microscopy and subcellular fractionation techniques. Glutathione S-transferase (GST) pull-down, immunoprecipitation and yeast two-hybrid screening were used to identify the SEPT11 interactome. Gene silencing was used to assess the role of SEPT11 in the regulation of insulin signalling and lipid metabolism in adipocytes. RESULTS: We demonstrate the expression of SEPT11 in human adipocytes and its upregulation in obese individuals, with SEPT11 mRNA content positively correlating with variables of insulin resistance in subcutaneous adipose tissue. SEPT11 content was regulated by lipogenic, lipolytic and proinflammatory stimuli in human adipocytes. SEPT11 associated with caveolae in mature adipocytes and interacted with both caveolin-1 and the intracellular fatty acid chaperone, fatty acid binding protein 5 (FABP5). Lipid loading of adipocytes caused the association of the three proteins with the surface of lipid droplets. SEPT11 silencing impaired insulin signalling and insulin-induced lipid accumulation in adipocytes. CONCLUSIONS/ INTERPRETATION: Our findings support a role for SEPT11 in lipid traffic and metabolism in adipocytes and open new avenues for research on the control of lipid storage in obesity and insulin resistance.
Authors: S Heinonen; L Saarinen; J Naukkarinen; A Rodríguez; G Frühbeck; A Hakkarainen; J Lundbom; N Lundbom; K Vuolteenaho; E Moilanen; P Arner; S Hautaniemi; A Suomalainen; J Kaprio; A Rissanen; K H Pietiläinen Journal: Int J Obes (Lond) Date: 2014-02-19 Impact factor: 5.095
Authors: Yi-Wei Huang; Ming Yan; Richard F Collins; Jessica E Diciccio; Sergio Grinstein; William S Trimble Journal: Mol Biol Cell Date: 2008-02-13 Impact factor: 4.138
Authors: Rocío Guzmán-Ruiz; Carmen Tercero-Alcázar; Yoana Rabanal-Ruiz; Alberto Díaz-Ruiz; Rajaa El Bekay; Oriol A Rangel-Zuñiga; M Carmen Navarro-Ruiz; Laura Molero; Antonio Membrives; Juan F Ruiz-Rabelo; Abhay Pandit; José López-Miranda; Francisco J Tinahones; María M Malagón Journal: FASEB J Date: 2020-04-15 Impact factor: 5.191