Attilio Marino1, Chiara Tonda-Turo2, Daniele De Pasquale3, Francesca Ruini4, Giada Genchi3, Simone Nitti2, Valentina Cappello5, Mauro Gemmi5, Virgilio Mattoli3, Gianluca Ciardelli4, Gianni Ciofani6. 1. Center for Micro-BioRobotics @SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy. Electronic address: attilio.marino@iit.it. 2. Nanobiotechnology Department, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy. 3. Center for Micro-BioRobotics @SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy. 4. Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy. 5. Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa, Italy. 6. Center for Micro-BioRobotics @SSSA, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025 Pontedera, Italy; Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy. Electronic address: gianni.ciofani@iit.it.
Abstract
BACKGROUND: The design of efficient nerve conduits able to sustain the axonal outgrowth and its guidance towards appropriate targets is of paramount importance in nerve tissue engineering. METHODS: In this work, we propose the preparation of highly aligned nanocomposite fibers of gelatin/cerium oxide nanoparticles (nanoceria), prepared by electrospinning. Nanoceria are powerful self-regenerative antioxidant nanomaterials, that behave as strong reactive oxygen species scavengers, and among various beneficial effects, they have been proven to inhibit the cell senescence and to promote the neurite sprouting. RESULTS: After a detailed characterization of the developed substrates, they have been tested on neuron-like SH-SY5Y cells, demonstrating strong antioxidant properties and beneficial multi-cue effects in terms of neurite development and alignment. CONCLUSIONS: Obtained findings suggest efficiency of the proposed substrates in providing combined topographical stimuli and antioxidant effects to cultured cells. GENERAL SIGNIFICANCE: Proposed nanocomposite scaffolds represent a promising approach for nerve tissue engineering and regenerative medicine.
BACKGROUND: The design of efficient nerve conduits able to sustain the axonal outgrowth and its guidance towards appropriate targets is of paramount importance in nerve tissue engineering. METHODS: In this work, we propose the preparation of highly aligned nanocomposite fibers of gelatin/cerium oxide nanoparticles (nanoceria), prepared by electrospinning. Nanoceria are powerful self-regenerative antioxidant nanomaterials, that behave as strong reactive oxygen species scavengers, and among various beneficial effects, they have been proven to inhibit the cell senescence and to promote the neurite sprouting. RESULTS: After a detailed characterization of the developed substrates, they have been tested on neuron-like SH-SY5Y cells, demonstrating strong antioxidant properties and beneficial multi-cue effects in terms of neurite development and alignment. CONCLUSIONS: Obtained findings suggest efficiency of the proposed substrates in providing combined topographical stimuli and antioxidant effects to cultured cells. GENERAL SIGNIFICANCE: Proposed nanocomposite scaffolds represent a promising approach for nerve tissue engineering and regenerative medicine.
Authors: Nicholas J Abuid; Morgan E Urdaneta; Kerim M Gattas-Asfura; Caterina Zientek; Cristina Isusi Silgo; Jose A Torres; Kevin J Otto; Cherie L Stabler Journal: Adv Nanobiomed Res Date: 2021-05-21
Authors: Mireille M J P E Sthijns; Clemens A van Blitterswijk; Vanessa L S LaPointe Journal: J Tissue Eng Regen Med Date: 2018-08-21 Impact factor: 3.963