Margaret E Gruen1, Kristen M Messenger2, Andrea E Thomson3, Emily H Griffith4, Hayley Paradise5, Shelly Vaden6, B D X Lascelles7. 1. Comparative Pain Research Program, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USA; Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27695, USA. Electronic address: megruen@ncsu.edu. 2. Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27695, USA; Department of Molecular and Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC, 27607, Raleigh, NC, USA. Electronic address: kmmessen@ncsu.edu. 3. Comparative Pain Research Program, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USA. Electronic address: aethomso@ncsu.edu. 4. Department of Statistics, North Carolina State University, 2311 Stinson Drive, Raleigh, NC 27695, USA. Electronic address: eghohmei@ncsu.edu. 5. Comparative Pain Research Program, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USA. 6. Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC, 27607, Raleigh, NC, USA. Electronic address: slvaden@ncsu.edu. 7. Comparative Pain Research Program, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USA; Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27695, USA; Center for Pain Research and Innovation, University of North Carolina School of Dentistry, Chapel Hill, NC 27599, USA. Electronic address: dxlascel@ncsu.edu.
Abstract
BACKGROUND: Degenerative joint disease (DJD) is highly prevalent in cats, and pain contributes to morbidity. In humans, alterations of cytokine concentrations have been associated with joint deterioration and pain. Similar changes have not been investigated in cats. Cytokine concentrations can be measured using multiplex technology with small samples of serum or plasma, however, serum and plasma are not interchangeable for most bioassays. Correlations for cytokine concentrations between serum and plasma have not been evaluated in cats. OBJECTIVE: To evaluate the levels of detection and agreement between serum and plasma samples in cats. ANIMALS: Paired serum and plasma samples obtained from 38 cats. METHODS: Blood was collected into anti-coagulant free and EDTA Vacutainer® tubes, serum or plasma extracted, and samples frozen at -80°C until testing. Duplicate samples were tested using a 19-plex feline cytokine/chemokine magnetic bead panel. RESULTS: Agreement between serum and plasma for many analytes was high, however correlation coefficients ranged from -0.01 to 0.97. Results from >50% of samples were below the lower limit of quantification for both serum and plasma for nine analytes, and for an additional three analytes for plasma only. CONCLUSIONS AND CLINICAL IMPORTANCE: While serum and plasma agreement was generally good, detection was improved using serum samples.
BACKGROUND:Degenerative joint disease (DJD) is highly prevalent in cats, and pain contributes to morbidity. In humans, alterations of cytokine concentrations have been associated with joint deterioration and pain. Similar changes have not been investigated in cats. Cytokine concentrations can be measured using multiplex technology with small samples of serum or plasma, however, serum and plasma are not interchangeable for most bioassays. Correlations for cytokine concentrations between serum and plasma have not been evaluated in cats. OBJECTIVE: To evaluate the levels of detection and agreement between serum and plasma samples in cats. ANIMALS: Paired serum and plasma samples obtained from 38 cats. METHODS: Blood was collected into anti-coagulant free and EDTA Vacutainer® tubes, serum or plasma extracted, and samples frozen at -80°C until testing. Duplicate samples were tested using a 19-plex feline cytokine/chemokine magnetic bead panel. RESULTS: Agreement between serum and plasma for many analytes was high, however correlation coefficients ranged from -0.01 to 0.97. Results from >50% of samples were below the lower limit of quantification for both serum and plasma for nine analytes, and for an additional three analytes for plasma only. CONCLUSIONS AND CLINICAL IMPORTANCE: While serum and plasma agreement was generally good, detection was improved using serum samples.
Authors: Kristin Skogstrand; Charlotte K Ekelund; Poul Thorsen; Ida Vogel; Bo Jacobsson; Bent Nørgaard-Pedersen; David M Hougaard Journal: J Immunol Methods Date: 2008-05-01 Impact factor: 2.303
Authors: Anil K Chaturvedi; Troy J Kemp; Ruth M Pfeiffer; Angelique Biancotto; Marcus Williams; Stella Munuo; Mark P Purdue; Ann W Hsing; Ligia Pinto; J Philip McCoy; Allan Hildesheim Journal: Cancer Epidemiol Biomarkers Prev Date: 2011-06-29 Impact factor: 4.254