Jiung-Pang Huang1, Mei-Ling Cheng1,2,3, Cheng-Yu Hung2, Chao-Hung Wang3, Po-Shiuan Hsieh4, Ming-Shi Shiao1,2, Jan-Kan Chen2,5, Dai-Er Li1, Li-Man Hung1,2,3,6. 1. Department of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan. 2. Center for Healthy and Aging Research, Chang Gung University, Taoyuan, Taiwan. 3. Division of Cardiology, Department of Internal Medicine, Heart Failure Center, Chang Gung Memorial Hospital, Keelung, Taiwan. 4. Department of Physiology and Biophysics, National Defense Medical Center, Taipei, Taiwan. 5. Department of Physiology and Pharmacology, College of Medicine, Chang Gung University, Taoyuan, Taiwan. 6. Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
Abstract
BACKGROUND: The aim of the present study was to compare insulin resistance and metabolic changes using a global lipidomic approach. METHODS: Rats were fed a high-fat diet (HFD) or a high-fructose diet (HFrD) for 12 weeks to induce insulin resistance (IR) syndrome. After 12 weeks feeding, physiological and biochemical parameters were examined. Insulin sensitivity and plasma metabolites were evaluated using a euglycemic-hyperinsulinemic clamp and mass spectrometry, respectively. Pearson's correlation coefficient was used to investigate the strength of correlations. RESULTS: Rats on both diets developed IR syndrome, characterized by hypertension, hyperlipidemia, hyperinsulinemia, impaired fasting glucose, and IR. Compared with HFrD-fed rats, non-esterified fatty acids were lower and body weight and plasma insulin levels were markedly higher in HFD-fed rats. Adiposity and plasma leptin levels were increased in both groups. However, the size of adipocytes was greater in HFD- than HFrD-fed rats. Notably, the lipidomic heat map revealed metabolites exhibiting greater differences in HFD- and HFrD-fed rats compared with controls. Plasma adrenic acid levels were higher in HFD- than HFrD-fed rats. Nevertheless, linoleic and arachidonic acid levels decreased in HFrD-fed rats compared with controls. Plasma concentrations of docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) were significantly reduced after feeding of both diets, particularly the HFrD. There was a strong positive correlation between these two fatty acids and the insulin sensitivity index. CONCLUSIONS: The systemic lipidomic analysis indicated that a reduction in DHA and DPA was strongly correlated with IR in rats under long-term overnutrition. These results provide a potential therapeutic target for IR and metabolic syndrome.
BACKGROUND: The aim of the present study was to compare insulin resistance and metabolic changes using a global lipidomic approach. METHODS:Rats were fed a high-fat diet (HFD) or a high-fructose diet (HFrD) for 12 weeks to induce insulin resistance (IR) syndrome. After 12 weeks feeding, physiological and biochemical parameters were examined. Insulin sensitivity and plasma metabolites were evaluated using a euglycemic-hyperinsulinemic clamp and mass spectrometry, respectively. Pearson's correlation coefficient was used to investigate the strength of correlations. RESULTS:Rats on both diets developed IR syndrome, characterized by hypertension, hyperlipidemia, hyperinsulinemia, impaired fasting glucose, and IR. Compared with HFrD-fed rats, non-esterified fatty acids were lower and body weight and plasma insulin levels were markedly higher in HFD-fed rats. Adiposity and plasma leptin levels were increased in both groups. However, the size of adipocytes was greater in HFD- than HFrD-fed rats. Notably, the lipidomic heat map revealed metabolites exhibiting greater differences in HFD- and HFrD-fed rats compared with controls. Plasma adrenic acid levels were higher in HFD- than HFrD-fed rats. Nevertheless, linoleic and arachidonic acid levels decreased in HFrD-fed rats compared with controls. Plasma concentrations of docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) were significantly reduced after feeding of both diets, particularly the HFrD. There was a strong positive correlation between these two fatty acids and the insulin sensitivity index. CONCLUSIONS: The systemic lipidomic analysis indicated that a reduction in DHA and DPA was strongly correlated with IR in rats under long-term overnutrition. These results provide a potential therapeutic target for IR and metabolic syndrome.
Authors: Haneen S Dwaib; Ghina Ajouz; Ibrahim AlZaim; Rim Rafeh; Ali Mroueh; Nahed Mougharbil; Marie-Elizabeth Ragi; Marwan Refaat; Omar Obeid; Ahmed F El-Yazbi Journal: J Am Heart Assoc Date: 2021-12-07 Impact factor: 6.106
Authors: Ola Al-Assi; Rana Ghali; Ali Mroueh; Abdullah Kaplan; Nahed Mougharbil; Ali H Eid; Fouad A Zouein; Ahmed F El-Yazbi Journal: Oxid Med Cell Longev Date: 2018-01-31 Impact factor: 6.543