Regulation of Cell Cycle Associated Genes by microRNA and Transcription Factor.

Cell cycle is a complex process and regulated at transcriptional, post-transcriptional and posttranslational levels. Large numbers of genes are implicated in the process. Abnormality at any stage of cell cycle may lead to diseases including cancer. To gain global view of genes associated with cell cycle, their regulation by transcription factors and microRNAs, we collected genes related to cell cycle from different databases. Experimentally validated targets of microRNAs are collected from miRTarbase. Transcription factors that bind to upstream sequences of cell cycle associated genes and microRNA genes were collected from published papers. We collected 3028 genes associated with cell cycle. These proteins belong to different protein classes like nucleic acid binding (594 proteins), transcription factors (305 proteins), cytoskeletal (232 proteins), kinases (174 proteins), phosphatase (111 proteins) and chaperones (84 proteins). Among 3028 cell cycle associated genes, 2125 genes are validated targets of 424 microRNAs; CDKN1A is a target of 46 miRNAs and miR-335 targets 301 genes. About 100 transcription factors had binding sites at potential promoter regions of 2722 genes and 329 microRNAs that target cell cycle associated genes. We presented the largest numbers of cell cycle associated genes. Many transcription factors regulate both cell cycle associated genes and the miRNAs that target cell cycle associated genes. These resources will be utilized to identify the co-regulation of cell cycle associated genes by transcription factors and miRNAs and to test specific hypothesis for cell cycle regulation and its alteration in different diseases.