Literature DB >> 27854365

Plug-and-playable fluorescent cell imaging modular toolkits using the bacterial superglue, SpyTag/SpyCatcher.

Hyojin Moon1, Yoonji Bae1, Hansol Kim1, Sebyung Kang1.   

Abstract

Simple plug-and-playable fluorescent cell imaging modular toolkits are established using the bacterial superglue SpyTag/SpyCatcher protein ligation system. A variety of affibody-fluorescent protein conjugates (AFPCs) are post-translationally generated via the isopeptide bond formation, and each AFPC effectively recognizes and binds to its targeting cells, visualizing them with selective colors on demand.

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Year:  2016        PMID： 27854365     DOI： 10.1039/c6cc07363h

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  7 in total

1.  A Magnetosome-Based Platform for Flow Biocatalysis.

Authors:  Esther Mittmann; Frank Mickoleit; Denis S Maier; Sabrina Y Stäbler; Marius A Klein; Christof M Niemeyer; Kersten S Rabe; Dirk Schüler
Journal:  ACS Appl Mater Interfaces       Date:  2022-05-04       Impact factor: 10.383

2.  Tagging and catching: rapid isolation and efficient labeling of organelles using the covalent Spy-System in planta.

Authors:  Martina Lang; Marlene Pröschel; Nico Brüggen; Uwe Sonnewald
Journal:  Plant Methods       Date:  2020-09-01       Impact factor: 4.993

3.  Covalent Protein Labeling by SpyTag-SpyCatcher in Fixed Cells for Super-Resolution Microscopy.

Authors:  Veronica Pessino; Y Rose Citron; Siyu Feng; Bo Huang
Journal:  Chembiochem       Date:  2017-06-22       Impact factor: 3.164

4.  Modular assembly of proteins on nanoparticles.

Authors:  Wenwei Ma; Angela Saccardo; Danilo Roccatano; Dorothy Aboagye-Mensah; Mohammad Alkaseem; Matthew Jewkes; Francesca Di Nezza; Mark Baron; Mikhail Soloviev; Enrico Ferrari
Journal:  Nat Commun       Date:  2018-04-16       Impact factor: 14.919

5.  Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox.

Authors:  Irsyad N A Khairil Anuar; Anusuya Banerjee; Anthony H Keeble; Alberto Carella; Georgi I Nikov; Mark Howarth
Journal:  Nat Commun       Date:  2019-04-15       Impact factor: 14.919

6.  Cell-permeable capsids as universal antigen carrier for the induction of an antigen-specific CD8+ T-cell response.

Authors:  Sami Akhras; Masako Toda; Klaus Boller; Kiyoshi Himmelsbach; Fabian Elgner; Marlene Biehl; Stephan Scheurer; Meike Gratz; Stefan Vieths; Eberhard Hildt
Journal:  Sci Rep       Date:  2017-08-29       Impact factor: 4.379

Review 7.  Catching a SPY: Using the SpyCatcher-SpyTag and Related Systems for Labeling and Localizing Bacterial Proteins.

Authors:  Daniel Hatlem; Thomas Trunk; Dirk Linke; Jack C Leo
Journal:  Int J Mol Sci       Date:  2019-04-30       Impact factor: 5.923
  7 in total

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