Christa Broholm1, Anders Henrik Olsson1, Alexander Perfilyev2, Linn Gillberg1, Ninna Schiøler Hansen1,3, Ashfaq Ali4, Brynjulf Mortensen5, Charlotte Ling2, Allan Vaag1,3,5. 1. Department of Endocrinology, Diabetes & Metabolism, Rigshospitalet, Copenhagen, Denmark. 2. Department of Clinical Sciences, Epigenetics & Diabetes Unit, Lund University Diabetes Centre, CRC, Malmö, Sweden. 3. Faculty of Health & Medical Sciences, University of Copenhagen, Copenhagen, Denmark. 4. Department of Clinical Sciences, GAME, Lund University Diabetes Centre, CRC, Malmö, Sweden. 5. Steno Diabetes Centre A/S, Gentofte, Denmark.
Abstract
AIM: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. METHODS: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. RESULTS: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. CONCLUSION: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.
AIM: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. METHODS: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. RESULTS: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. CONCLUSION:Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.
Entities:
Keywords:
DNA methylation; adipogenesis; adiponectin; epigenetics; stem cells
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