Literature DB >> 27854017

Analytical Ultracentrifuge Analysis of Nucleosomes Assembled from Recombinant, Acid-Extracted, HPLC-Purified Histones.

Manjinder S Cheema1, Juan Ausió2.   

Abstract

The accumulating discoveries of new posttranslational modifications (PTMs) and the increasing relevance of histone variants within the frame of epigenetics demand the availability of methods for a rapid and efficient nucleosome reconstitution to analyze their structural and functional implications. Here we describe a method suitable for this purpose, starting from bacterially expressed histones, solubilized by acid and purified by reversed-phase high-performance liquid chromatography. This method allows the preparation of micrograms to milligram amounts of in vitro-assembled nucleosomes. Finally, we demonstrate the efficiency of this method for the structural analysis of nucleosomes in the analytical ultracentrifuge.

Keywords:  Acid extraction; Analytical ultracentrifuge (AUC); Fast protein liquid chromatography (FPLC); Nucleosome; Recombinant histones; Reversed-phase high-performance liquid chromatography (HPLC)

Mesh：

Substances：

Year:  2017        PMID： 27854017     DOI： 10.1007/978-1-4939-6630-1_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

1.  NSD2 dimethylation at H3K36 promotes lung adenocarcinoma pathogenesis.

Authors:  Deepanwita Sengupta; Liyong Zeng; Yumei Li; Simone Hausmann; Debopam Ghosh; Gang Yuan; Thuyen N Nguyen; Ruitu Lyu; Marcello Caporicci; Ana Morales Benitez; Garry L Coles; Vladlena Kharchenko; Iwona Czaban; Dulat Azhibek; Wolfgang Fischle; Mariusz Jaremko; Ignacio I Wistuba; Julien Sage; Łukasz Jaremko; Wei Li; Pawel K Mazur; Or Gozani
Journal:  Mol Cell       Date:  2021-09-22       Impact factor: 17.970

2.  Molecular and Biochemical Methods Useful for the Epigenetic Characterization of Chromatin-Associated Proteins in Bivalve Molluscs.

Authors:  Ciro Rivera-Casas; Rodrigo Gonzalez-Romero; Rafael A Garduño; Manjinder S Cheema; Juan Ausio; Jose M Eirin-Lopez
Journal:  Front Physiol       Date:  2017-08-08       Impact factor: 4.566

3.  Elevated NSD3 histone methylation activity drives squamous cell lung cancer.

Authors:  Gang Yuan; Natasha M Flores; Simone Hausmann; Shane M Lofgren; Vladlena Kharchenko; Maria Angulo-Ibanez; Deepanwita Sengupta; Xiaoyin Lu; Iwona Czaban; Dulat Azhibek; Silvestre Vicent; Wolfgang Fischle; Mariusz Jaremko; Bingliang Fang; Ignacio I Wistuba; Katrin F Chua; Jack A Roth; John D Minna; Ning-Yi Shao; Łukasz Jaremko; Pawel K Mazur; Or Gozani
Journal:  Nature       Date:  2021-02-03       Impact factor: 69.504
  3 in total

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