Literature DB >> 2785060

Rapid assessment of the antitetanus immune status of a subject using Dot-ELISA.

P Mastroeni1, M S Leonardi, D Gazzara, B Bizzini.   

Abstract

We describe a Dot-ELISA system for the rapid, specific and reliable assessment of a subject's antitetanus immune status. The conditions for Dot-ELISA were selected to give positive reactions only for sera with antitetanus antibody titers equal to or higher than 0.06 I.U./ml, considered to represent protection when using "in vitro" methods. This Dot-ELISA method could advantageously be applied to the monitoring of vaccination campaigns, as well as for assessing the antitetanus immune status of the wounded.

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Year:  1989        PMID: 2785060     DOI: 10.1007/bf00145053

Source DB:  PubMed          Journal:  Eur J Epidemiol        ISSN: 0393-2990            Impact factor:   8.082


  12 in total

1.  Comparison of the rotazyme assay with an avidin-biotin-amplified dot-immunobinding assay for detecting rotaviruses.

Authors:  D Reynolds; J H Hughes
Journal:  J Infect Dis       Date:  1985-09       Impact factor: 5.226

2.  A hand-held photometer for quantitative dot-immunobinding assays.

Authors:  C Bordier; P Ryter
Journal:  Anal Biochem       Date:  1986-01       Impact factor: 3.365

3.  A dot-immunobinding assay for monoclonal and other antibodies.

Authors:  R Hawkes; E Niday; J Gordon
Journal:  Anal Biochem       Date:  1982-01-01       Impact factor: 3.365

4.  Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.

Authors:  F C Kafatos; C W Jones; A Efstratiadis
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

5.  [Immunogenic property of tetanus anatoxins of various degrees of purity and polymerization].

Authors:  B Bizzini; A Turpin; M Raynaud
Journal:  Ann Inst Pasteur (Paris)       Date:  1971-06

6.  Quantitation of immunoglobulin E antibody to cytomegalovirus by antibody capture enzyme-linked immunosorbent assay.

Authors:  A M van Loon; J T van der Logt; F W Heessen; J van der Veen
Journal:  J Clin Microbiol       Date:  1985-04       Impact factor: 5.948

7.  Dot-enzyme immunoassay for visual detection of antibodies to pseudorabies virus in swine serum.

Authors:  A Afshar; P F Wright; G C Dulac
Journal:  J Clin Microbiol       Date:  1986-03       Impact factor: 5.948

8.  Nylon bead enzyme-linked immunosorbent assay for detection of sub-picogram quantities of Brucella antigens.

Authors:  V Y Perera; M T Creasy; A J Winter
Journal:  J Clin Microbiol       Date:  1983-09       Impact factor: 5.948

9.  Immunodot assay for determining the isotype and light chain type of murine monoclonal antibodies in unconcentrated hybridoma culture supernates.

Authors:  J S McDougal; S W Browning; S Kennedy; D D Moore
Journal:  J Immunol Methods       Date:  1983-10-28       Impact factor: 2.303

10.  Reduced false positive reactions in the Dot-enzyme-linked immunosorbent assay for human visceral leishmaniasis.

Authors:  M G Pappas; R Hajkowski; D B Tang; W T Hockmeyer
Journal:  Clin Immunol Immunopathol       Date:  1985-03
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