| Literature DB >> 27847975 |
Genhui Xiao1,2,3, Zongfu Wu1,2,3, Shouming Zhang1,2,3, Huanyu Tang1,2,3, Fengqiu Wang1,2,3, Chengping Lu4,5,6.
Abstract
Streptococcus suis is a major pathogen of pigs and also an important zoonotic agent for humans. A S. suis protein containing Mac-1 domain (designated Mac) is a protective antigen, exclusively cleaves porcine IgM, and contributes to complement evasion with the presence of high titers of specific porcine anti-S. suis IgM, but its role in S. suis virulence has not been investigated in natural healthy host without specific IgM. In this study, a mac deletion mutant was constructed by homologous recombination in S. suis serotype 2 virulent reference strain P1/7. Deletion of mac did not significantly influence phagocytosis or intracellular survival within murine macrophages RAW264.7, or the oxidative-burst induction of RAW264.7 and murine neutrophils. Furthermore, the mutant is as virulent as the wild-type strain in pig, mouse, and zebrafish infection models. Our data suggest that Mac is not essential for S. suis virulence in strain P1/7 in natural healthy host without specific IgM, and the immunogenicity of Mac does not appear to correlate with its significance for virulence.Entities:
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Year: 2016 PMID： 27847975 DOI： 10.1007/s00284-016-1160-3
Source DB: PubMed Journal: Curr Microbiol ISSN： 0343-8651 Impact factor: 2.188