Milan Swadas1, Bhavna Dave2, Soham M Vyas3, Nupur Shah3. 1. Senior Lecturer, Department of Pedodontics and Preventive Dentistry Narsinhbhai Patel Dental College and Hospital, Visnagar Gujarat, India. 2. Professor and Head, Department of Pedodontics and Preventive Dentistry, KM Shah Dental College and Hospital, Sumadeep Vidyapeeth University, Vadodara, Gujarat, India. 3. Postgraduate Student, Department of Pedodontics and Preventive Dentistry, KM Shah Dental College and Hospital, Sumadeep Vidyapeeth University, Vadodara, Gujarat, India.
Abstract
INTRODUCTION: Streptococcus mutans has been implicated as primary microorganisms which cause dental caries in humans. There has been an increased interest in the therapeutic properties of some medicinal plants and natural compounds which have demonstrated antibacterial activities. Grape is one of the plants of this group which contains tannin and polyphenolic compound. AIM: To evaluate and compare antibacterial activity of grape seed extract at different concentrations with chlorhexidine gluconate against S. mutans. MATERIALS AND METHODS: Grape seeds were extracted with ethanol/water ratio of 70:30 volume/volume. The extracts were filtered through Whatman No. 1 filter paper until it becomes colorless. Streptococcus mutans strains were taken. To check the antimicrobial properties of grape seed extract at different concentration and chlorhexidine gluconate, they were added to S. mutans strain and incubated for 48 hours than colony-forming units/mL were checked. RESULTS: Grape seed extract at higher concentration were found to be more potent against S. mutans. Chlorhexidine gluconate was found to have most potent antibacterial action compared to all different concentrations of grape seed extract. CONCLUSION: Grape seed extract as a natural antimicrobial compound has inhibitory effect against S. mutans. HOW TO CITE THIS ARTICLE: Swadas M, Dave B, Vyas SM, Shah N. Evaluation and Comparison of the Antibacterial Activity against Streptococcus mutans of Grape Seed Extract at Different Concentrations with Chlorhexidine Gluconate: An in vitro Study. Int J Clin Pediatr Dent 2016;9(3):181-185.
INTRODUCTION:Streptococcus mutans has been implicated as primary microorganisms which cause dental caries in humans. There has been an increased interest in the therapeutic properties of some medicinal plants and natural compounds which have demonstrated antibacterial activities. Grape is one of the plants of this group which contains tannin and polyphenolic compound. AIM: To evaluate and compare antibacterial activity of grape seed extract at different concentrations with chlorhexidine gluconate against S. mutans. MATERIALS AND METHODS: Grape seeds were extracted with ethanol/water ratio of 70:30 volume/volume. The extracts were filtered through Whatman No. 1 filter paper until it becomes colorless. Streptococcus mutans strains were taken. To check the antimicrobial properties of grape seed extract at different concentration and chlorhexidine gluconate, they were added to S. mutans strain and incubated for 48 hours than colony-forming units/mL were checked. RESULTS: Grape seed extract at higher concentration were found to be more potent against S. mutans. Chlorhexidine gluconate was found to have most potent antibacterial action compared to all different concentrations of grape seed extract. CONCLUSION: Grape seed extract as a natural antimicrobial compound has inhibitory effect against S. mutans. HOW TO CITE THIS ARTICLE: Swadas M, Dave B, Vyas SM, Shah N. Evaluation and Comparison of the Antibacterial Activity against Streptococcus mutans of Grape Seed Extract at Different Concentrations with Chlorhexidine Gluconate: An in vitro Study. Int J Clin Pediatr Dent 2016;9(3):181-185.
Authors: Juan Pablo Loyola-Rodriguez; Rita Elizabeth Martinez-Martinez; Blanca Irma Flores-Ferreyra; Nuria Patiño-Marin; Angel Gabriel Alpuche-Solis; Juan Francisco Reyes-Macias Journal: J Clin Pediatr Dent Date: 2008 Impact factor: 1.065
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