Xiaobei Deng1, Nannan Feng2, Min Zheng3, Xiaofei Ye4, Hongyan Lin5, Xiao Yu6, Zhihua Gan7, Zheng Fang8, Huan Zhang9, Ming Gao10, Zhi-Jie Zheng11, Herbert Yu12, Wenjun Ding13, Biyun Qian14. 1. Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: dengxiaobei@126.com. 2. Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: fengnan214@163.com. 3. Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: zhengmin8010@126.com. 4. Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: fly0770@163.com. 5. Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: cara-chris@sjtu.edu.cn. 6. Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: yuxiao19910101@163.com. 7. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address: zhgan@mail.buct.edu.cn. 8. Department of Anaesthesiology, Huadong Hospital, Fudan University, Shanghai 200040, China. Electronic address: shiningbluedan@hotmail.com. 9. Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China. Electronic address: brenda1919@163.com. 10. Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China. Electronic address: gaoming68@aliyun.com. 11. Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: zheng22762@icloud.com. 12. Cancer Epidemiology Program, University of Hawaii Cancer Center, 701 Ilalo Street, Honolulu, HI 96813, USA. Electronic address: hyu@cc.hawaii.edu. 13. Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China. Electronic address: dingwj@ucas.ac.cn. 14. Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: qianbiyun@shsmu.edu.cn.
Abstract
BACKGROUND: Evidence shows that individuals who are under long-term exposure to environmental PM2.5 are at increased risk of lung cancer. Various laboratory experiments also suggest several mechanistic links between PM2.5 exposure and lung carcinogenesis. However, a long non-coding RNA (lncRNA) mediated pathogenic change after PM2.5 exposure and its potential roles in tumorigenesis and disease progression have not been reported. METHODS: Cytotoxicity induced by PM2.5 was assessed by using scanning electron microscopy and transmission electron microscopy. ROS generation, autophagy, and metastasis induced by PM2.5 were detected by using comprehensive approaches. Expression of lncRNA-loc146880 and lc3b (autophagy marker) in A549 cells, lung tissue and serum were determined by RT-PCR and Western blotting. RESULTS: PM2.5 could be internalized into lung cancer cells, resulting in marked increases in ROS levels and autophagy. ROS may be responsible for increased expression of loc146880 which further up-regulates autophagy. Both loc146880 and autophagy could promote lung tumor cell migration, invasion and EMT. In addition, a positive correlation was observed between loc146880 expression and lc3b levels in tumor tissues and serum of lung cancer patients. CONCLUSION: Taken together, our data suggest that PM2.5 exposure induces ROS, which activates loc146880 expression. The lncRNA, in turn, up-regulates autophagy and promotes the malignant behaviors of lung cancer cells. GENERAL SIGNIFICANCE: The results show the toxicological effects of PM2.5 in lung tumor progression and metastasis.
BACKGROUND: Evidence shows that individuals who are under long-term exposure to environmental PM2.5 are at increased risk of lung cancer. Various laboratory experiments also suggest several mechanistic links between PM2.5 exposure and lung carcinogenesis. However, a long non-coding RNA (lncRNA) mediated pathogenic change after PM2.5 exposure and its potential roles in tumorigenesis and disease progression have not been reported. METHODS:Cytotoxicity induced by PM2.5 was assessed by using scanning electron microscopy and transmission electron microscopy. ROS generation, autophagy, and metastasis induced by PM2.5 were detected by using comprehensive approaches. Expression of lncRNA-loc146880 and lc3b (autophagy marker) in A549 cells, lung tissue and serum were determined by RT-PCR and Western blotting. RESULTS: PM2.5 could be internalized into lung cancer cells, resulting in marked increases in ROS levels and autophagy. ROS may be responsible for increased expression of loc146880 which further up-regulates autophagy. Both loc146880 and autophagy could promote lung tumor cell migration, invasion and EMT. In addition, a positive correlation was observed between loc146880 expression and lc3b levels in tumor tissues and serum of lung cancerpatients. CONCLUSION: Taken together, our data suggest that PM2.5 exposure induces ROS, which activates loc146880 expression. The lncRNA, in turn, up-regulates autophagy and promotes the malignant behaviors of lung cancer cells. GENERAL SIGNIFICANCE: The results show the toxicological effects of PM2.5 in lung tumor progression and metastasis.
Authors: Michelle C Turner; Zorana J Andersen; Andrea Baccarelli; W Ryan Diver; Susan M Gapstur; C Arden Pope; Diddier Prada; Jonathan Samet; George Thurston; Aaron Cohen Journal: CA Cancer J Clin Date: 2020-08-25 Impact factor: 508.702
Authors: Catarina Albuquerque; Rita Manguinhas; João G Costa; Nuno Gil; Jordi Codony-Servat; Matilde Castro; Joana P Miranda; Ana S Fernandes; Rafael Rosell; Nuno G Oliveira Journal: Transl Lung Cancer Res Date: 2021-06