| Literature DB >> 27832512 |
Ke Zhang1, Huidong Li2, Wuxi Chen1, Minli Zhao1, Haiyang Cui1, Qingsong Min1, Haijun Wang1, Shulin Chen1, Demao Li3.
Abstract
Docosapentaenoic acid/docosahexaenoic acid ratio (DPA/DHA ratio) in Schizochytrium was relatively stable. But ideally the ratio of DPA/DHA will vary according to the desired end use. This study reports several ways of modulating the DPA/DHA ratio. Incubation times changed the DPA/DHA ratio, and changes in this ratio were associated with the variations in the saturated fatty acid (SFAs) content. Propionic acid sharply increased the SFAs content in lipids, dramatically decreased the even-chain SFAs content, and reduced the DPA/DHA ratio. Pentanoic acid (C5:0) and heptanoic acid (C7:0) had similar effects as propionic acid, whereas butyric acid (C4:0), hexanoic acid (C6:0), and octanoic acid (C8:0) did not change the fatty acid profile and the DPA/DHA ratio. Transcription analyses show that β-oxidation might be responsible for this phenomenon. Iodoacetamide upregulated polyunsaturated fatty acid (PUFA) synthase genes, reduced the DHA content, and improved the DPA content, causing the DPA/DHA ratio to increase. These results present new insights into the regulation of the DPA/DHA ratio.Entities:
Keywords: Docosapentaenoic acid; Docosapentaenoic acid / docosahexaenoic acid ratio; Docosapentaenoic acid biosynthesis; Polyunsaturated fatty acid synthase; Schizochytrium
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Year: 2016 PMID： 27832512 DOI： 10.1007/s12010-016-2311-5
Source DB: PubMed Journal: Appl Biochem Biotechnol ISSN： 0273-2289 Impact factor: 2.926