Nirmal Marasini1, Ashwini K Giddam1, Zeinab G Khalil2,3, Waleed M Hussein1, Robert J Capon2, Michael R Batzloff4, Michael F Good4, Istvan Toth1,2,5, Mariusz Skwarczynski1. 1. School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia. 2. Institute for Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia. 3. Diamantina Institute, The University of Queensland, Woolloongabba, QLD, 4102, Australia. 4. Institute for Glycomics, Griffith University, Gold Coast, QLD 4222, Australia. 5. School of Pharmacy, The University of Queensland, Woolloongabba, QLD 4102, Australia.
Abstract
AIM: To develop novel polymer-based nanoscale delivery system for lipopeptide-based vaccine against group A Streptococcus (GAS). MATERIALS & METHODS: Four types of lipopeptide antigen-loaded polymeric nanoparticles (NP) were prepared. NP were accessed for their capacity to be taken up by dendritic cells; effect on dendritic cell maturation; ability to induce mucosal and systemic immunity; and capability to induce antibody responses that opsonize GAS bacteria. RESULTS & DISCUSSION: The combination of adjuvanting properties of lipopeptides and dextran/trimethyl chitosan-based NP had a synergistic effect on humoral immunity, and the produced antibodies showed high opsonic activity against clinical GAS isolates. CONCLUSION: Biocompatible NP-bearing trimethyl chitosan and dextran are efficient as mucosal adjuvants for the intranasal delivery of lipopeptide-based vaccines.
AIM: To develop novel polymer-based nanoscale delivery system for lipopeptide-based vaccine against group A Streptococcus (GAS). MATERIALS & METHODS: Four types of lipopeptide antigen-loaded polymeric nanoparticles (NP) were prepared. NP were accessed for their capacity to be taken up by dendritic cells; effect on dendritic cell maturation; ability to induce mucosal and systemic immunity; and capability to induce antibody responses that opsonize GAS bacteria. RESULTS & DISCUSSION: The combination of adjuvanting properties of lipopeptides and dextran/trimethyl chitosan-based NP had a synergistic effect on humoral immunity, and the produced antibodies showed high opsonic activity against clinical GAS isolates. CONCLUSION: Biocompatible NP-bearing trimethyl chitosan and dextran are efficient as mucosal adjuvants for the intranasal delivery of lipopeptide-based vaccines.
Entities:
Keywords:
Group A Streptococcus; chitosan; dextran; lipopeptides; nasal; vaccine
Authors: Nirmal Marasini; Changkui Fu; Nicholas L Fletcher; Christopher Subasic; Gerald Er; Karine Mardon; Kristofer J Thurecht; Andrew K Whittaker; Lisa M Kaminskas Journal: Nanomaterials (Basel) Date: 2020-12-08 Impact factor: 5.076
Authors: David Wibowo; Sytze H T Jorritsma; Zennia Jean Gonzaga; Benjamin Evert; Shuxiong Chen; Bernd H A Rehm Journal: Biomaterials Date: 2020-12-10 Impact factor: 12.479