| Literature DB >> 27830558 |
Serene M L Lee1, Tobias S Schiergens1,2, Maresa Demmel1,3, Reinhard M K Thasler2, Wolfgang E Thasler4,5.
Abstract
Co-transplantation of hepatocytes and hepatic stellate cells has been shown to increase the engraftment of transplanted hepatocytes in the liver. Here, we describe a method for the simultaneous isolation of human primary hepatocytes and hepatic stellate cells from the same donor for co-transplantation or for use in in vitro cell culture models.Entities:
Keywords: Collagenase; Hepatic stellate cells; Hepatocyte isolation; Hepatocytes; Human; Human hepatocytes; Liver; Liver cell isolation; Perfusion; Stellate cell isolation
Mesh：
Year: 2017 PMID： 27830558 DOI： 10.1007/978-1-4939-6506-9_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745