| Literature DB >> 27826854 |
Marta Muñoz-Barrera1, Fernando Monje-Casas2.
Abstract
The budding index and the morphology of the spindle and the nucleus are excellent markers for the analysis of the progression through the different stages of the cell cycle in Saccharomyces cerevisiae. Here, we describe a protocol to evaluate the budding index in this model organism using phase contrast microscopy. We also describe an indirect immunofluorescence method designed for the visualization of microtubules and the nucleus in S. cerevisiae. Finally, we explain how both methodologies can be used in order to analyze cell cycle progression in the budding yeast.Entities:
Keywords: Budding index; Cell cycle; Immunofluorescence; Microtubules; Mitotic exit; Nuclear morphology; Spindle
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Year: 2017 PMID： 27826854 DOI： 10.1007/978-1-4939-6502-1_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745