| Literature DB >> 27822863 |
Rolf H A M Vossen1, Stefan J White2.
Abstract
Droplet digital PCR (ddPCR) is based on the isolated amplification of thousands of individual DNA molecules simultaneously, with each molecule compartmentalized in a droplet. The presence of amplified product in each droplet is indicated by a fluorescent signal, and the proportion of positive droplets allows the precise quantification of a given sequence. In this chapter we briefly outline the basis of ddPCR, and describe two different applications using the Bio-Rad QX200 system: genotyping copy number variation and quantification of Illumina sequencing libraries.Entities:
Keywords: Copy number variation; DNA quantitation; Digital PCR; NGS
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Year: 2017 PMID： 27822863 DOI： 10.1007/978-1-4939-6442-0_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745