| Literature DB >> 27822854 |
Abstract
High-resolution melting analysis (HRMA) is a simple, quick, and effective method to scan and screen PCR amplicons for sequence variants. HRMA is a nondestructive closed tube assay; after PCR, DNA melting can directly be performed on the amplified samples without any purification or separation steps. For single SNP genotyping, HRMA is an attractive alternative to Sanger sequencing, restriction enzyme analysis, and hydrolysis probes.Keywords: DNA; Melting curve analysis; Single nucleotide polymorphism; Variant detection
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Year: 2017 PMID： 27822854 DOI： 10.1007/978-1-4939-6442-0_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745