Genomic polymorphisms in a Laboratory Isolate of Mycobacterium tuberculosis Reference Strain H37Rv (ATCC27294).

The complete genome sequence of Mycobacterium tuberculosis reference strain H37Rv (ATCC27294) was determined on an isolate carried in our laboratory collection for almost 20 years and named H37RvSiena. DNA sequence analysis showed that the genome of H37RvSiena was 4,410,911 bp in size and contained 101 genetic polymorphisms compared to H37Rv: 83 single nucleotide polymorphisms, 10 insertions, and 8 deletions of which one was 617-bp long and seven ranged from 1 to 7 bp. Comparison with the genomes of two other H37Rv derivatives allowed identification of 28 polymorphisms specific for H37RvSiena.