| Literature DB >> 27815870 |
Noah Fine1, Oriyah Barzilay2, Michael Glogauer2.
Abstract
Neutrophils are primary phagocytes that recognize their targets through surface chemistry, either through Pattern Recognition Receptor (PPR) interaction with Pathogen-Associated Molecular Patterns (PAMPs) or through immunoglobulin (Ig) or complement mediated recognition. Opsonization can be important for target recognition, and phagocytosis by neutrophils in whole blood can be greatly enhanced due to the presence of blood serum components and platelets. Powerful and sensitive flow cytometry based methods are presented to measure phagocytosis by human blood neutrophils and mouse peritoneal neutrophils.Entities:
Keywords: Flow cytometry; Neutrophil; Peritonitis; Phagocytosis; pHrodo
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Year: 2017 PMID： 27815870 DOI： 10.1007/978-1-4939-6581-6_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745