Sanipon Chanburee1,2, Waree Tiyaboonchai1,2,3. 1. a Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences , Naresuan University , Phitsanulok , Thailand. 2. b The Center of Excellence for Innovation in Chemistry (PERCH-CIC) , Commission on Higher Education, Ministry of Education , Bangkok , Thailand. 3. c The Center of Excellence in Medical Biotechnology , Naresuan University , Phitsanulok , Thailand.
Abstract
PURPOSE: To examine effects of polymer types on the mucoadhesive properties of polymer-coated nanostructured lipid carriers (NLCs). Experiment: Curcumin-loaded NLCs were prepared using a warm microemulsion technique followed by coating particle surface with mucoadhesive polymers: polyethylene glycol400 (PEG400), polyvinyl alcohol (PVA), and chitosan (CS). The physicochemical properties and entrapment efficacy were examined. In vitro mucoadhesive studies were assessed by wash-off test. In addition, the stability of mucoadhesive NLCs in gastrointestinal fluids and the pattern of drug release were also investigated. FINDINGS: The obtained nanoparticles showed spherical shape with size ranging between 200 nm and 500 nm and zeta potential between -37 and -9 mV depending on the type of polymer coating. Up to 80% drug entrapment efficacy was observed. In vitro mucoadhesive studies revealed that PEG-NLCs and PVA-NLCs were adhered strongly to freshly porcine intestinal mucosa, more than 2-fold mucoadhesive compared to CS-NLCs and uncoated-NLCs. The particle size of all polymer-coated NLCs could be maintained in both simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) suggesting good physical stability in physiological fluid. In contrast, uncoated-NLCs showed particle aggregation in SGF. In vitro dissolution studies revealed a fast release characteristic.
PURPOSE: To examine effects of polymer types on the mucoadhesive properties of polymer-coated nanostructured lipid carriers (NLCs). Experiment: Curcumin-loaded NLCs were prepared using a warm microemulsion technique followed by coating particle surface with mucoadhesive polymers: polyethylene glycol400 (PEG400), polyvinyl alcohol (PVA), and chitosan (CS). The physicochemical properties and entrapment efficacy were examined. In vitro mucoadhesive studies were assessed by wash-off test. In addition, the stability of mucoadhesive NLCs in gastrointestinal fluids and the pattern of drug release were also investigated. FINDINGS: The obtained nanoparticles showed spherical shape with size ranging between 200 nm and 500 nm and zeta potential between -37 and -9 mV depending on the type of polymer coating. Up to 80% drug entrapment efficacy was observed. In vitro mucoadhesive studies revealed that PEG-NLCs and PVA-NLCs were adhered strongly to freshly porcine intestinal mucosa, more than 2-fold mucoadhesive compared to CS-NLCs and uncoated-NLCs. The particle size of all polymer-coated NLCs could be maintained in both simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) suggesting good physical stability in physiological fluid. In contrast, uncoated-NLCs showed particle aggregation in SGF. In vitro dissolution studies revealed a fast release characteristic.
Authors: Guo Yu; Zakir Ali; Anam Sajjad Khan; Kalim Ullah; Humzah Jamshaid; Alam Zeb; Muhammad Imran; Sadia Sarwar; Han-Gon Choi; Fakhar Ud Din Journal: Int J Nanomedicine Date: 2021-05-11
Authors: Davide Allegrini; Raffaele Raimondi; Alfredo Borgia; Tania Sorrentino; Giovanni Montesano; Panos Tsoutsanis; Giuseppe Cancian; Yash Verma; Francesco Paolo De Rosa; Mario R Romano Journal: Int J Mol Sci Date: 2022-03-24 Impact factor: 5.923