S A Koppes1,2, P Kemperman3,4, I Van Tilburg1, F Calkoen-Kwa1, K A Engebretsen5, G J Puppels6,7, P J Caspers6,7, S Kezic1. 1. a Coronel Institute of Occupational Health, Academic Medical Center, University of Amsterdam , Amsterdam , The Netherlands. 2. b Department of Dermatology-Allergology , VU University Medical Center , Amsterdam , The Netherlands. 3. c Department of Dermatology , Academic Medical Center, University of Amsterdam , Amsterdam , The Netherlands. 4. d Department of Dermatology , Waterlandziekenhuis , Purmerend , The Netherlands. 5. e National Allergy Research Centre, Department of Dermato-Allergology , Herlev and Gentofte University Hospital, University of Copenhagen , Hellerup , Denmark. 6. f Department of Dermatology , Erasmus University Rotterdam , Rotterdam , The Netherlands. 7. g RiverD International B.V , Rotterdam , The Netherlands.
Abstract
BACKGROUND: Natural moisturizing factor (NMF) is used as genotypic and phenotypic biomarker in diagnostics. This study is a side-to-side comparison of two different methods to determine NMF in atopic dermatitis patients: Raman microspectroscopy and stratum corneum tape stripping followed by HPLC. RESULTS: Measured NMF values were significantly correlated (R2 = .61; p < .0001), both methods demonstrated a concentration-depth dependence of NMF and reduced NMF levels in the carriers of filaggrin null mutations. Good agreement between measurements of left and right arms indicated robustness and good reproducibility of both methods. CONCLUSIONS: Both methods showed comparable performance, choice of method will rather be influenced by practical consideration.
BACKGROUND: Natural moisturizing factor (NMF) is used as genotypic and phenotypic biomarker in diagnostics. This study is a side-to-side comparison of two different methods to determine NMF in atopic dermatitispatients: Raman microspectroscopy and stratum corneum tape stripping followed by HPLC. RESULTS: Measured NMF values were significantly correlated (R2 = .61; p < .0001), both methods demonstrated a concentration-depth dependence of NMF and reduced NMF levels in the carriers of filaggrin null mutations. Good agreement between measurements of left and right arms indicated robustness and good reproducibility of both methods. CONCLUSIONS: Both methods showed comparable performance, choice of method will rather be influenced by practical consideration.
Entities:
Keywords:
Dermatology; HPCL and Raman microspectroscopy; NMF
Authors: Ewa Izabela Podobas; Danuta Gutowska-Owsiak; Sébastien Moretti; Jarosław Poznański; Mariusz Kulińczak; Marcin Grynberg; Aleksandra Gruca; Arkadiusz Bonna; Dawid Płonka; Graham Ogg; Wojciech Bal Journal: Front Mol Biosci Date: 2022-03-10
Authors: Skaidre Jankovskaja; Maxim Morin; Anna Gustafsson; Chris D Anderson; Boglarka Lehoczki; Johan Engblom; Sebastian Björklund; Melinda Rezeli; György Marko-Varga; Tautgirdas Ruzgas Journal: Anal Chem Date: 2022-04-08 Impact factor: 8.008