Literature DB >> 2780540

Amino acid substitutions that increase the thermal stability of the lambda Cro protein.

A A Pakula1, R T Sauer.   

Abstract

A mutant Cro protein, which bears the Ile-30----Leu substitution, is thermally unstable and degraded more rapidly than wild-type Cro in vivo. Using an antibody screen, we have isolated five different second site suppressor substitutions that reduce the proteolytic hypersensitivity of this mutant Cro protein. Two of the suppressor substitutions increase the thermal stability of Cro by 12 degrees C to 14 degrees C. These amino acid substitutions affect residues 16 and 26, which are substantially exposed to solvent in the crystal structure of wild-type Cro.

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Year:  1989        PMID： 2780540     DOI： 10.1002/prot.340050303

Source DB:  PubMed          Journal:  Proteins        ISSN： 0887-3585


  16 in total

1.  Retroevolution of lambda Cro toward a stable monomer.

Authors:  Kelly R LeFevre; Matthew H J Cordes
Journal:  Proc Natl Acad Sci U S A       Date:  2003-02-21       Impact factor: 11.205

2.  The stability of extracellular beta-glucosidase from Aspergillus niger is significantly enhanced by non-covalently attached polysaccharides.

Authors:  M H Rashid; K S Siddiqui
Journal:  Folia Microbiol (Praha)       Date:  1996       Impact factor: 2.099

3.  Increasing protein stability: importance of DeltaC(p) and the denatured state.

Authors:  Hailong Fu; Gerald Grimsley; J Martin Scholtz; C Nick Pace
Journal:  Protein Sci       Date:  2010-05       Impact factor: 6.725

4.  Directed evolution methods for overcoming trade-offs between protein activity and stability.

Authors:  Samuel D Stimple; Matthew D Smith; Peter M Tessier
Journal:  AIChE J       Date:  2019-10-09       Impact factor: 3.993

5.  Nonlinear protein degradation and the function of genetic circuits.

Authors:  Nicolas E Buchler; Ulrich Gerland; Terence Hwa
Journal:  Proc Natl Acad Sci U S A       Date:  2005-06-22       Impact factor: 11.205

6.  Structure-based identification of catalytic residues.

Authors:  Ran Yahalom; Dan Reshef; Ayana Wiener; Sagiv Frankel; Nir Kalisman; Boaz Lerner; Chen Keasar
Journal:  Proteins       Date:  2011-04-12

7.  Protein-DNA conformational changes in the crystal structure of a lambda Cro-operator complex.

Authors:  R G Brennan; S L Roderick; Y Takeda; B W Matthews
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

Review 8.  Conformational stability of dimeric proteins: quantitative studies by equilibrium denaturation.

Authors:  K E Neet; D E Timm
Journal:  Protein Sci       Date:  1994-12       Impact factor: 6.725

9.  A highly thermostable neutral protease from Bacillus caldolyticus: cloning and expression of the gene in Bacillus subtilis and characterization of the gene product.

Authors:  B van den Burg; H G Enequist; M E van der Haar; V G Eijsink; B K Stulp; G Venema
Journal:  J Bacteriol       Date:  1991-07       Impact factor: 3.490

10.  Enhanced stability in vivo of a thermodynamically stable mutant form of yeast iso-1-cytochrome c.

Authors:  D A Pearce; F Sherman
Journal:  Mol Gen Genet       Date:  1995-11-15
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