| Literature DB >> 27804286 |
Manon Ripoll, Patrick Neuberg, Antoine Kichler, Nassera Tounsi1, Alain Wagner, Jean-Serge Remy.
Abstract
A novel generation of pH-responsive photopolymerized diacetylenic amphiphile (PDA) micelles with a diameter of 10 nm was designed and optimized for the intracellular delivery of siRNAs. Dialysis and photopolymerization of the micelles allowed a strong reduction of the cytotoxicity of the nanovector, while the hydrophilic histidine headgroup permitted enhancing the siRNA delivery potential by improving the endosomal escape via imidazole protonation. These PDA-micellar systems were fully characterized by DLS, TEM, and DOSY-NMR experiments. The resulting bioactive complexes of PDA-micelles with siRNA were shown to have an optimal size below 100 nm.Entities:
Keywords: imidazole; micelles; photopolymerization; proton sponge effect; siRNA
Mesh：
Substances：
Year: 2016 PMID： 27804286 DOI： 10.1021/acsami.6b09365
Source DB: PubMed Journal: ACS Appl Mater Interfaces ISSN： 1944-8244 Impact factor: 9.229