| Literature DB >> 27801513 |
Rubén Martínez-Barricarte1, Sarah Jill de Jong1, Janet Markle1, Roel de Paus2, Stephanie Boisson-Dupuis1,3,4, Jacinta Bustamante3,4, Esther van de Vosse2, Bernhard Fleckenstein5, Jean-Laurent Casanova1,3,4,6,7.
Abstract
Human T cells can be transformed and expanded with herpesvirus saimiri (HVS). HVS-transformed T cells from patients have facilitated the study of a broad range of primary immunodeficiencies (PID) in which T-cell development or function is altered. However, the utility of HVS-transformed T cells for genetic studies has been limited by technical challenges in the expression of exogenous genes, including wild-type or mutant alleles. A novel, gamma retrovirus-based method for the simple and reliable transduction, purification, and study of HVS-transformed T cells is described. © 2016 by John Wiley & Sons, Inc.Entities:
Keywords: HVS-transformed T cell; T cells; herpesvirus saimiri-transformed T cells; retrovirus; transduction
Mesh:
Year: 2016 PMID: 27801513 DOI: 10.1002/cpim.15
Source DB: PubMed Journal: Curr Protoc Immunol ISSN: 1934-3671