Literature DB >> 27796959

Techniques for the Reprogramming of Exogenous Stem/Progenitor Cell Populations Towards a Mammary Epithelial Cell Fate.

Gilbert H Smith1, Corinne A Boulanger2.   

Abstract

This chapter considers the techniques necessary and required for the reprogramming of exogenous stem/progenitor cell populations towards a mammary epithelial cell fate. The protocols describe how to isolate cells from alternate mouse organs such as testicles of male mice and mix them with mammary cells to generate chimeric glands comprised of male and female epithelial cells that are fully competent. During the reformation of mammary stem cell niches by dispersed epithelial cells, in the context of the intact epithelium-free mammary stroma, non-mammary cells are sequestered and reprogrammed to perform mammary epithelial cell functions including those ascribed to mammary stem/progenitor cells. This therefore is a powerful technique for the redirection of cells from other organs/cancer cells to a normal mammary phenotype.

Entities:  

Keywords:  Fat pad clearing; Immunohistochemistry; Mammary gland; Mouse mammary; Reprogramming; Survival surgery; Transplantation

Mesh：

Year:  2017        PMID： 27796959      PMCID： PMC6690038          DOI： 10.1007/978-1-4939-6475-8_14

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  14 in total

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Authors:  S B Rasmussen; E Kordon; R Callahan; G H Smith
Journal:  Oncogene       Date:  2001-08-30       Impact factor: 9.867

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Authors:  Linheng Li; Ting Xie
Journal:  Annu Rev Cell Dev Biol       Date:  2005       Impact factor: 13.827

4.  The in vivo life span of normal and preneoplastic mouse mammary glands: a serial transplantation study.

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Journal:  Proc Natl Acad Sci U S A       Date:  1968-09       Impact factor: 11.205

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Authors:  C W Daniel; L J Young
Journal:  Exp Cell Res       Date:  1971-03       Impact factor: 3.905

6.  The influence of host and tissue age on life span and growth rate of serially transplanted mouse mammary gland.

Authors:  L J Young; D Medina; K B DeOme; C W Daniel
Journal:  Exp Gerontol       Date:  1971-02-01       Impact factor: 4.032

7.  Quantitative polymerase chain reaction-based assay with fluorogenic Y-chromosome specific probes to measure bone marrow chimerism in mice.

Authors:  Stefan Oliver Peters; Kathrin Bauermeister; Jan Philipp Simon; Biggi Branke; Thomas Wagner
Journal:  J Immunol Methods       Date:  2002-02-01       Impact factor: 2.303

8.  Long-term in vivo expression of genes introduced by retrovirus-mediated transfer into mammary epithelial cells.

Authors:  G H Smith; D Gallahan; J A Zwiebel; S M Freeman; R H Bassin; R Callahan
Journal:  J Virol       Date:  1991-11       Impact factor: 5.103

9.  GROWTH OF MOUSE MAMMARY GLANDS IN VIVO AFTER MONOLAYER CULTURE.

Authors:  C W DANIEL; K B DEOME
Journal:  Science       Date:  1965-08-06       Impact factor: 47.728

10.  An adjunct mammary epithelial cell population in parous females: its role in functional adaptation and tissue renewal.

Authors:  Kay-Uwe Wagner; Corinne A Boulanger; MaLinda D Henry; Magdalene Sgagias; Lothar Hennighausen; Gilbert H Smith
Journal:  Development       Date:  2002-03       Impact factor: 6.868
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