Literature DB >> 2779516

Identification of nucleotides critical for activity of the Pseudomonas putida catBC promoter.

T L Aldrich1, R K Rothmel, A M Chakrabarty.   

Abstract

Pseudomonas putida utilizes the catBC operon, which encodes cis,cis-muconate lactonizing enzyme I (MLEI; EC 5.5.1.1) and muconolactone isomerase (MI; EC 5.3.3.4), for growth on benzoate as a sole carbon source. This operon is positively regulated, and the promoter is located 64 bp upstream of the catB translational start site. Using site-specific mutagenesis, we identified nucleotides that influenced the induction of this promoter. Promoter activity was monitored with the promoter probe vector pKT240. Transcription of mRNA from mutant promoters was determined by primer extension mapping. Comparison of the initiation start site of mutant promoters with that of the wild-type promoter identified a single functional promoter.

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Year:  1989        PMID： 2779516     DOI： 10.1007/BF00331277

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  31 in total

1.  Homology between nucleotide sequences of promoter regions of nah and sal operons of NAH7 plasmid of Pseudomonas putida.

Authors:  M A Schell
Journal:  Proc Natl Acad Sci U S A       Date:  1986-01       Impact factor: 11.205

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Journal:  Genetics       Date:  1970-10       Impact factor: 4.562

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Authors:  A M Cook; H Grossenbacher; R Hütter
Journal:  Experientia       Date:  1983-11-15

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Authors:  S Inouye; Y Asai; A Nakazawa; T Nakazawa
Journal:  J Bacteriol       Date:  1986-06       Impact factor: 3.490

5.  Mutational analysis of the lac regulatory region: second-site changes that activate mutant promoters.

Authors:  R K Rothmel; J E LeClerc
Journal:  Nucleic Acids Res       Date:  1989-05-25       Impact factor: 16.971

6.  Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.

Authors:  D H Figurski; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1979-04       Impact factor: 11.205

7.  Identification of the promoter of the Pseudomonas gene coding for carboxypeptidase G2.

Authors:  N P Minton; L E Clarke
Journal:  J Mol Appl Genet       Date:  1985

8.  Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template.

Authors:  M J Zoller; M Smith
Journal:  DNA       Date:  1984-12

9.  Determination of the transcription initiation site and identification of the protein product of the regulatory gene xylR for xyl operons on the TOL plasmid.

Authors:  S Inouye; A Nakazawa; T Nakazawa
Journal:  J Bacteriol       Date:  1985-09       Impact factor: 3.490

10.  Nucleotide sequence and transcriptional initiation site of two Pseudomonas aeruginosa pilin genes.

Authors:  K Johnson; M L Parker; S Lory
Journal:  J Biol Chem       Date:  1986-11-25       Impact factor: 5.157
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  10 in total

Review 1.  Biodegradation of halogenated organic compounds.

Authors:  G R Chaudhry; S Chapalamadugu
Journal:  Microbiol Rev       Date:  1991-03

2.  Nucleotide sequencing and characterization of Pseudomonas putida catR: a positive regulator of the catBC operon is a member of the LysR family.

Authors:  R K Rothmel; T L Aldrich; J E Houghton; W M Coco; L N Ornston; A M Chakrabarty
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

3.  Functional analysis of the Pseudomonas putida regulatory protein CatR: transcriptional studies and determination of the CatR DNA-binding site by hydroxyl-radical footprinting.

Authors:  R K Rothmel; D L Shinabarger; M R Parsek; T L Aldrich; A M Chakrabarty
Journal:  J Bacteriol       Date:  1991-08       Impact factor: 3.490

4.  Characterization of the Pseudomonas sp. strain P51 gene tcbR, a LysR-type transcriptional activator of the tcbCDEF chlorocatechol oxidative operon, and analysis of the regulatory region.

Authors:  J R van der Meer; A C Frijters; J H Leveau; R I Eggen; A J Zehnder; W M de Vos
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

5.  Sequence analysis of the Pseudomonas sp. strain P51 tcb gene cluster, which encodes metabolism of chlorinated catechols: evidence for specialization of catechol 1,2-dioxygenases for chlorinated substrates.

Authors:  J R van der Meer; R I Eggen; A J Zehnder; W M de Vos
Journal:  J Bacteriol       Date:  1991-04       Impact factor: 3.490

6.  Regulation of iron assimilation: nucleotide sequence analysis of an iron-regulated promoter from a fluorescent pseudomonad.

Authors:  D J O'Sullivan; F O'Gara
Journal:  Mol Gen Genet       Date:  1991-08

7.  Organization and sequence analysis of the 2,4-dichlorophenol hydroxylase and dichlorocatechol oxidative operons of plasmid pJP4.

Authors:  E J Perkins; M P Gordon; O Caceres; P F Lurquin
Journal:  J Bacteriol       Date:  1990-05       Impact factor: 3.490

8.  Up-promoter mutations in the trpBA operon of Pseudomonas aeruginosa.

Authors:  C Y Han; I P Crawford; C S Harwood
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

9.  Roles of CatR and cis,cis-muconate in activation of the catBC operon, which is involved in benzoate degradation in Pseudomonas putida.

Authors:  M R Parsek; D L Shinabarger; R K Rothmel; A M Chakrabarty
Journal:  J Bacteriol       Date:  1992-12       Impact factor: 3.490

10.  Localization and functional analysis of structural and regulatory dehalogenase genes carried on DEH from Pseudomonas putida PP3.

Authors:  A W Thomas; A W Topping; J H Slater; A J Weightman
Journal:  J Bacteriol       Date:  1992-03       Impact factor: 3.490
  10 in total

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