Fatemeh Mohseni 1 , Safar Farajnia 2 , Mahdieh Abbasalizad Farhangi 3 , Manouchehr Khoshbaten 2 , Mohammad-Asghari Jafarabadi 4 Show Affiliations »
Abstract
OBJECTIVE: To investigate the association of uncoupling protein-2 (UCP2) -866G > A gene polymorphism (rs659366) with nonalcoholic fatty liver disease (NAFLD). METHODS: We performed a case-control study with a cohort of 75 patients with NAFLD (of Iranian ethnicity) and 76 healthy individuals of Iranian ethnicity. The UCP2 -866G > A polymorphism (rs659366) was determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). RESULTS: Patients with AA and AG genotypes were 71% and 68%, respectively, more likely to have NAFLD, compared with individuals with the GG genotype (reference group). In subjects with a GG genotype, serum triglyceride (TG) concentration was significantly higher in patients with NAFLD (P = .04). Serum alanine aminotransferase (ALT) concentrations in all 3 genotypes and serum aspartate aminotransferase (AST) concentrations in AG and GG genotypes of UCP2 gene polymorphism were significantly higher in patients (P <.05). CONCLUSION: Our results revealed a modest modifier effect of -866G>A UCP2 polymorphism in patients with NAFLD. © American Society for Clinical Pathology, 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
OBJECTIVE: To investigate the association of uncoupling protein-2 (UCP2 ) -866G > A gene polymorphism (rs659366 ) with nonalcoholic fatty liver disease (NAFLD). METHODS: We performed a case-control study with a cohort of 75 patients with NAFLD (of Iranian ethnicity) and 76 healthy individuals of Iranian ethnicity. The UCP2 -866G > A polymorphism (rs659366 ) was determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). RESULTS: Patients with AA and AG genotypes were 71% and 68%, respectively, more likely to have NAFLD, compared with individuals with the GG genotype (reference group). In subjects with a GG genotype, serum triglyceride (TG ) concentration was significantly higher in patients with NAFLD (P = .04). Serum alanine aminotransferase (ALT) concentrations in all 3 genotypes and serum aspartate aminotransferase (AST ) concentrations in AG and GG genotypes of UCP2 gene polymorphism were significantly higher in patients (P <.05). CONCLUSION: Our results revealed a modest modifier effect of -866G>A UCP2 polymorphism in patients with NAFLD. © American Society for Clinical Pathology, 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Entities: Chemical
Disease
Gene
Mutation
Species
Keywords:
anthropometric indices; biochemical parameters; nonalcoholic fatty liver disease (NAFLD); uncoupling protein 2 (UCP2) gene; –866G>A polymorphism
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Year: 2016
PMID： 27794526 DOI： 10.1093/labmed/lmw052
Source DB: PubMed Journal: Lab Med ISSN： 0007-5027