Denis Gümbel1,2, Nadine Gelbrich3, Martin Weiss4, Matthias Napp3, Georg Daeschlein5, Axel Sckell3, Stephan A Ender6, Axel Kramer7, Martin Burchardt4, Axel Ekkernkamp3,2, Matthias B Stope4. 1. Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University of Medicine Greifswald, Greifswald, Germany denis.guembel@uni-greifswald.de. 2. Department of Trauma and Orthopaedic Surgery, BG Klinikum Unfallkrankenhaus Berlin gGmbH, Berlin, Germany. 3. Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University of Medicine Greifswald, Greifswald, Germany. 4. Department of Urology, University of Medicine Greifswald, Greifswald, Germany. 5. Department of Dermatology, University of Medicine Greifswald, Greifswald, Germany. 6. Department of Orthopaedics and Orthopaedic Surgery, University of Medicine Greifswald, Greifswald, Germany. 7. Department of Hygiene and Environmental Medicine, University of Medicine Greifswald, Greifswald, Germany.
Abstract
BACKGROUND/AIM: Cold atmospheric plasma has been shown to inhibit tumor cell growth and induce tumor cell death. The aim of the study was to investigate the effects of cold atmospheric plasma treatment on proliferation of human osteosarcoma cells and to characterize the underlying cellular mechanisms. MATERIALS AND METHODS: Human osteosarcoma cells (U2-OS and MNNG/HOS) were treated with cold atmospheric plasma and seeded in culture plates. Cell proliferation, p53 and phospho-p53 protein expression and nuclear morphology were assessed. RESULTS: The treated human osteosarcoma cell lines exhibited attenuated proliferation rates by up to 66%. The cells revealed an induction of p53, as well as phospho-p53 expression, by 2.3-fold and 4.5-fold, respectively, compared to controls. 4',6-diamidino-2-phenylindole staining demonstrated apoptotic nuclear condensation following cold atmospheric plasma treatment. CONCLUSION: Cold atmospheric plasma treatment significantly attenuated cell proliferation in a preclinical in vitro osteosarcoma model. The resulting increase in p53 expression and phospho-activation in combination with characteristic nuclear changes indicate this was through induction of apoptosis. Copyright
BACKGROUND/AIM: Cold atmospheric plasma has been shown to inhibit tumor cell growth and induce tumor cell death. The aim of the study was to investigate the effects of cold atmospheric plasma treatment on proliferation of humanosteosarcoma cells and to characterize the underlying cellular mechanisms. MATERIALS AND METHODS:Humanosteosarcoma cells (U2-OS and MNNG/HOS) were treated with cold atmospheric plasma and seeded in culture plates. Cell proliferation, p53 and phospho-p53 protein expression and nuclear morphology were assessed. RESULTS: The treated humanosteosarcoma cell lines exhibited attenuated proliferation rates by up to 66%. The cells revealed an induction of p53, as well as phospho-p53 expression, by 2.3-fold and 4.5-fold, respectively, compared to controls. 4',6-diamidino-2-phenylindole staining demonstrated apoptotic nuclear condensation following cold atmospheric plasma treatment. CONCLUSION: Cold atmospheric plasma treatment significantly attenuated cell proliferation in a preclinical in vitro osteosarcoma model. The resulting increase in p53 expression and phospho-activation in combination with characteristic nuclear changes indicate this was through induction of apoptosis. Copyright
Authors: Josephine M Jacoby; Silas Strakeljahn; Andreas Nitsch; Sander Bekeschus; Peter Hinz; Alexander Mustea; Axel Ekkernkamp; Mladen V Tzvetkov; Lyubomir Haralambiev; Matthias B Stope Journal: Int J Mol Sci Date: 2020-06-23 Impact factor: 5.923
Authors: Lyubomir Haralambiev; Andreas Nitsch; Josephine M Jacoby; Silas Strakeljahn; Sander Bekeschus; Alexander Mustea; Axel Ekkernkamp; Matthias B Stope Journal: Int J Mol Sci Date: 2020-03-26 Impact factor: 5.923
Authors: Lyubomir Haralambiev; Lasse Wien; Nadine Gelbrich; Jörn Lange; Sinan Bakir; Axel Kramer; Martin Burchardt; Axel Ekkernkamp; Denis Gümbel; Matthias B Stope Journal: Oncol Lett Date: 2019-11-19 Impact factor: 2.967
Authors: Andreas Nitsch; Silas Strakeljahn; Josephine M Jacoby; Konrad F Sieb; Alexander Mustea; Sander Bekeschus; Axel Ekkernkamp; Matthias B Stope; Lyubomir Haralambiev Journal: Biomedicines Date: 2022-03-17