Zahra Rezaie1,1, Mohammad Taheri2,3,1, Leila Kohan1, Arezou Sayad2. 1. Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran. 2. Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 3. Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Shahid Labbafi Nejad Educational Hospital, Tehran, Iran.
Abstract
BACKGROUND: Multiple sclerosis (MS) as a complex neurological disease can be due to vitamin D deficiency. CYP27B1 is referred to as a vitamin D metabolizing enzyme. MATERIALS AND METHODS: This study compared the expression level of CYP27B1 in Relapsing-Remitting MS (RRMS) patients with normal individuals in Iran. The RNA was extracted from 50 RRMS patients and 50 normal controls. Quantitative RT-PCR was adopted to measure the expression level of CYP27B1 gene. RESULTS: The expression level of CYP27B1gene was significantly lower in the RRMS patients than their normal counterparts (P value = 0.04). Also, the RRMS females participating had a significant reduction in CYP27B1 gene expression compared to normal females (P-Value = 0.01). In addition, the correlation between CYP27B1 expression level, and the risk of Expanded Disability Status Scale of Kurtzke (EDSS) was not linear. Additionally, there was no significant correlation between expression status of CYP27B1gene and duration of the disease. CONCLUSION: A significant decrease in the expression level of CYP27A1 in female patients could indicate their greater vulnerability to MS than the male patients.
BACKGROUND:Multiple sclerosis (MS) as a complex neurological disease can be due to vitamin D deficiency. CYP27B1 is referred to as a vitamin D metabolizing enzyme. MATERIALS AND METHODS: This study compared the expression level of CYP27B1 in Relapsing-Remitting MS (RRMS) patients with normal individuals in Iran. The RNA was extracted from 50 RRMS patients and 50 normal controls. Quantitative RT-PCR was adopted to measure the expression level of CYP27B1 gene. RESULTS: The expression level of CYP27B1gene was significantly lower in the RRMS patients than their normal counterparts (P value = 0.04). Also, the RRMS females participating had a significant reduction in CYP27B1 gene expression compared to normal females (P-Value = 0.01). In addition, the correlation between CYP27B1 expression level, and the risk of Expanded Disability Status Scale of Kurtzke (EDSS) was not linear. Additionally, there was no significant correlation between expression status of CYP27B1gene and duration of the disease. CONCLUSION: A significant decrease in the expression level of CYP27A1 in female patients could indicate their greater vulnerability to MS than the male patients.
Entities:
Keywords:
CYP27B1; expression; multiple sclerosis; real time PCR
Authors: Delores J Grant; Ani Manichaikul; Anthony J Alberg; Elisa V Bandera; Jill Barnholtz-Sloan; Melissa Bondy; Michele L Cote; Ellen Funkhouser; Patricia G Moorman; Lauren C Peres; Edward S Peters; Ann G Schwartz; Paul D Terry; Xin-Qun Wang; Temitope O Keku; Cathrine Hoyo; Andrew Berchuck; Dale P Sandler; Jack A Taylor; Katie M O'Brien; Digna R Velez Edwards; Todd L Edwards; Alicia Beeghly-Fadiel; Nicolas Wentzensen; Celeste Leigh Pearce; Anna H Wu; Alice S Whittemore; Valerie McGuire; Weiva Sieh; Joseph H Rothstein; Francesmary Modugno; Roberta Ness; Kirsten Moysich; Mary Anne Rossing; Jennifer A Doherty; Thomas A Sellers; Jennifer B Permuth-Way; Alvaro N Monteiro; Douglas A Levine; Veronica Wendy Setiawan; Christopher A Haiman; Loic LeMarchand; Lynne R Wilkens; Beth Y Karlan; Usha Menon; Susan Ramus; Simon Gayther; Aleksandra Gentry-Maharaj; Kathryn L Terry; Daniel W Cramer; Ellen L Goode; Melissa C Larson; Scott H Kaufmann; Rikki Cannioto; Kunle Odunsi; John L Etter; Ruea-Yea Huang; Marcus Q Bernardini; Alicia A Tone; Taymaa May; Marc T Goodman; Pamela J Thompson; Michael E Carney; Shelley S Tworoger; Elizabeth M Poole; Diether Lambrechts; Ignace Vergote; Adriaan Vanderstichele; Els Van Nieuwenhuysen; Hoda Anton-Culver; Argyrios Ziogas; James D Brenton; Line Bjorge; Helga B Salvensen; Lambertus A Kiemeney; Leon F A G Massuger; Tanja Pejovic; Amanda Bruegl; Melissa Moffitt; Linda Cook; Nhu D Le; Angela Brooks-Wilson; Linda E Kelemen; Paul D P Pharoah; Honglin Song; Ian Campbell; Diana Eccles; Anna DeFazio; Catherine J Kennedy; Joellen M Schildkraut Journal: Cancer Med Date: 2019-04-18 Impact factor: 4.711