Literature DB >> 277916

Soluble biospecific macromolecule for purification of estrogen receptor.

P Hubert, J Mester, E Dellacherie, J Neel, E E Baulieu.   

Abstract

An alternative to affinity chromatography purification of proteins based on the use of a water-soluble biospecific polymer has been applied to the estrogen receptor from calf uterus. The receptor (4S-trypsin form) was bound to a dextran-estradiol conjugate (molecular weight approximately 500,000) and the complex was isolated by gel filtration. Highly purified receptor was subsequently released from the dextran-estradiol conjugate by exchange with [3H]estradiol.

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Year:  1978        PMID： 277916      PMCID： PMC392730          DOI： 10.1073/pnas.75.7.3143

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  11 in total

1.  Affinity chromatography of the uterine estradiol receptor on estradiol-PAB-cellulose: an artefact.

Authors:  M Best-Belpomme; H Richard-Foy; C Secco-Millet; E E Baulieu
Journal:  Biochimie       Date:  1976       Impact factor: 4.079

2.  Production and detection of antibodies against the estrogen receptor from calf uterine cytosol.

Authors:  L L Fox; G Redeuilh; P Baskevitch; E E Baulieu; H Richard-Foy
Journal:  FEBS Lett       Date:  1976-03-15       Impact factor: 4.124

3.  Progesterone binding components of chick oviduct. X. Purification by affinity chromatography.

Authors:  R W Kuhn; W T Schrader; R G Smith; B W O'Malley
Journal:  J Biol Chem       Date:  1975-06-10       Impact factor: 5.157

4.  Affinity chromatography and the purification of estrogen receptors.

Authors:  V Sica; I Parikh; E Nola; G A Puca; P Cuatrecasas
Journal:  J Biol Chem       Date:  1973-09-25       Impact factor: 5.157

5.  Parameters influencing the purification of calf uterus estrogen 'receptor' by affinity chromatography.

Authors:  H Truong; E E Baulieu
Journal:  FEBS Lett       Date:  1974-09-15       Impact factor: 4.124

Review 6.  Affinity chromatography.

Authors:  P Cuatrecasas
Journal:  Annu Rev Biochem       Date:  1971       Impact factor: 23.643

7.  Purification and allosteric properties of yeast pyruvate kinase.

Authors:  R Haeckel; B Hess; W Lauterborn; K H Wüster
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1968-05

8.  Criteria for affinity chromatography of steroid-binding macromolecules.

Authors:  J H Ludens; J R DeVries; D D Fanestil
Journal:  J Biol Chem       Date:  1972-12-10       Impact factor: 5.157

9.  Determination of high-affinity oestrogen receptor sites in uterine supernatant preparations.

Authors:  J Méster; D M Rbertson; P Feherty; A E Kellie
Journal:  Biochem J       Date:  1970-12       Impact factor: 3.857

10.  Human erythrocyte pyruvate kinase. Its purification and some properties.

Authors:  G E Staal; J F Koster; H Kamp; L van Milligen-Boersma; C Veeger
Journal:  Biochim Biophys Acta       Date:  1971-01-13
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  2 in total

1.  Enhancement of the efficacy of an antagonist of an extracellular receptor by attachment to the surface of a biocompatible carrier.

Authors:  Charles A Wartchow; Susan E Alters; Pamela D Garzone; Lingyun Li; Steven Choi; Neal E DeChene; Tina Doede; Linong Huang; John S Pease; Zhimin Shen; Susan J Knox; Jeffrey L Cleland
Journal:  Pharm Res       Date:  2004-10       Impact factor: 4.200

2.  Immunohistologic and histochemical methods for detection of steroid binding in breast cancer: a reappraisal.

Authors:  L P Pertschuk; E H Tobin; A C Carter; K B Eisenberg; V C Leo; E Gaetjens; N D Bloom
Journal:  Breast Cancer Res Treat       Date:  1981       Impact factor: 4.872
  2 in total

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