Reappraisal of the clinical use of leflunomide in rheumatoid arthritis and psoriatic arthritis.

Leflunomide is a disease-modifying antirheumatic drug (DMARD) that has been in routine clinical use for the treatment of rheumatoid arthritis (RA) and psoriatic arthritis for a decade. In RA, clinical trials of up to two years' duration showed that leflunomide monotherapy was equivalent to methotrexate in clinical and radiographic disease outcomes (tender and swollen joint counts, physician and patient global assessments, American College of Rheumatology and Disease Activity Score responses, slowing or halting of radiographic progression). In a number of studies, quality of life measurements indicated that leflunomide is superior to methotrexate. Leflunomide has been studied in combination with methotrexate and shows efficacy in patients only partly responsive to this agent. Recent trials have shown that leflunomide can be used safely with biologic DMARDs, including antitumor necrosis factor agents and rituximab as part of the treatment algorithm in place of methotrexate as a cotherapy. Leflunomide has demonstrated efficacy as a monotherapy in psoriatic arthritis, and it also has a beneficial effect in psoriasis. Postmarketing studies have shown that retention on treatment with leflunomide is equal to methotrexate and superior to other DMARDs. In general, its side effect profile is acceptable compared with other DMARDS, with nausea, diarrhea, and hair fall occurring commonly, but only rarely leading to discontinuation. Liver toxicity is the most significant problem in clinical use although it is uncommon. Peripheral neuropathy, hypertension, pneumonitis, and cytopenia occur more rarely. Leflunomide is contraindicated in pregnancy and should be used with caution in women during child-bearing years. In this review, the place of leflunomide in therapy is discussed and practical advice informed by evidence is given regarding dosing regimens, safety monitoring, and managing side effects. Leflunomide remains one of the most useful of the nonbiologic DMARDs.

Intorduction

Leflunomide was last the subject of a review of clinical use in 20061 and has more recently been the subject of a benefit-risk analysis.2 Major changes in disease management have occurred, with a focus on achieving remission in inflammatory arthritis through frequent assessment driving treatment changes, use of combinations of disease-modifying antirheumatic drug (DMARD) therapies and the early use of biologic agents. The challenges in treating rheumatoid arthritis (RA) and psoriatic arthritis relate to the unpredictability of response to treatment, the frequent occurrence of dose-limiting side effects, and rarer serious adverse reactions requiring cessation of treatment. There remains an unmet need for a greater range of DMARDs, and for drugs that provide an alternative to methotrexate as baseline therapy, particularly for use in combination with biologic DMARDs, such as antitumor necrosis factor α (anti-TNF) agents and rituximab. In many countries, access to these very expensive drugs is limited, and the use of traditional DMARDs, including leflunomide, remains a key strategy. This review incorporates new information about the clinical use of leflunomide in RA and psoriatic arthritis, places it in the context of existing knowledge of the drug and takes an evidence-based practice approach to give practical advice regarding the use of leflunomide in clinical situations.

Chemistry, pharmacology, and mechanism of action

Leflunomide is an immunomodulatory and anti-inflammatory drug initially developed and tested in animal models of autoimmunity and transplant medicine.3 It is an isoxazole derivative, freely soluble in water, and almost completely absorbed by the gut. Leflunomide is a prodrug having little or no immunomodulatory activity until nonenzymatic conversion to the active metabolite, teriflunomide (A77 1726), probably within the gut wall and liver.4 In the plasma it is highly (>99%) protein-bound. It demonstrates linear pharmacokinetics in doses up to 100 mg, with peak plasma concentrations of teriflunomide being reached in 6–12 hours.5 The plasma half-life is approximately 15 (range 5–40) days,6 but this is reduced to 1–2 days by cholestyramine or activated charcoal washout, implying significant enterohepatic recirculation. This can result in significant plasma levels being maintained for up to two years after stopping treatment. Teriflunomide is an inhibitor of cytochrome P450 2C9 in vitro, conferring a risk of drug interactions, particularly with warfarin, phenytoin, and tolbutamide.5 Rifampicin increases teriflunomide levels about 40% by an unknown mechanism, and leflunomide displaces nonsteroidal anti-inflammatory drugs (NSAIDs) from plasma proteins. The clinical significance of these effects is uncertain.5 Leflunomide is excreted in roughly equal proportions in urine and bile, but teriflunomide levels are not affected significantly by hemodialysis.7 There is no evidence of accumulation in renal failure, although the free fraction of teriflunomide is increased, and so dose reduction should not be needed. However, studies have not addressed this issue, and caution is advised when using leflunomide in renal impairment.5

Leflunomide has a weak uricosuric effect in the proximal tubule, and significant falls in serum uric acid were observed in trials.8 Hyperuricemia has been linked to hypertension in RA,9 but paradoxically, despite its urate-lowering effect, leflunomide is associated with an increase in blood pressure. The mechanism of this effect is unknown but it may be renally mediated, possibly through an effect on anion exchange in the proximal tubule, an increase in sympathetic drive, or by increasing the salt and water retention caused by NSAIDs.

The principal mechanism of action is by inhibition of the mitochondrial enzyme dihydro-orotic acid dehydrogenase (DHODH), which catalyses a key step in the de novo metabolic pathway of pyrimidine synthesis. T lymphocytes depend on this to supply the large increase in ribonucleic acid precursors necessary for their proliferation. As a result, lymphocytes become depleted of ribonucleotide precursors (principally rUMP) reducing DNA and RNA synthesis and resulting in the arrest of cell proliferation in the G1 phase of the cell cycle.10,11 Other body cells (eg, gastrointestinal, hemopoietic) are able to use the salvage pathway as a supply of uridine for pyrimidine synthesis, which probably explains the relative lack of unwanted effects of leflunomide on cell proliferation in other body systems, such as cytopenia or mucositis.12 The relevance of this mechanism of action is supported by pharmacogenomic studies showing that people with a common missense polymorphism of the DHODH gene sequence have lower remission rates on leflunomide than those with the usual allele.13 Polymorphism of the DHOHD gene is also implicated in the cytochrome P1A2-mediated liver toxicity of leflunomide.14

A multitude of additional mechanisms of action have been proposed, including the inhibition of tyrosine kinases,15 reduction in growth factor synthesis and interleukin 2,16 inhibition of lymphocyte adhesion to synovial high endothelial venules,17 inhibition of neutrophil and macrophage migration,18 inhibition of adhesion molecules and matrix metalloproteinases,19–21 and reduction in phospholipase A2 activation products, such as prostaglandins and leukotrienes.3 While some of these mechanisms have been shown to operate in synovial tissue, most are thought unlikely to be significant contributors to the mechanism of action at the tissue concentrations achieved by the doses used for clinical indications.

Clinical studies in rheumatoid arthritis

The great majority of clinical studies have been conducted in RA, and there has been only one randomized controlled trial of leflunomide in psoriatic arthritis. The justification for the use of leflunomide in this condition and in other forms of inflammatory polyarthritis follows a heuristic argument. A 2003 Cochrane review of six clinical trials in RA,22 updated in 2009 with 33 studies published to June 2008,23 concluded that “leflunomide improves all clinical outcomes and delays radiographic progression at both six and 12 months compared with placebo”, and commented that “its efficacy and adverse events at 2 years of treatment are comparable to sulfasalazine or methotrexate”. It was not possible to tell whether treatment outcomes were better or worse than those obtained with sulfasalazine or methotrexate because no significant differences were seen in the primary clinical outcome measures between the active treatment groups in the clinical trials.

Leflunomide as monotherapy

The efficacy of leflunomide in RA was tested in a Phase II trial6 and established in three double-blind, randomized, 6–12 month, Phase III clinical trials.8,24–26 It was compared with methotrexate25 and sulfasalazine8 in two placebo-controlled trials and with methotrexate in an active comparator trial against methotrexate.24 The sulfasalazine trial lasted six months and the methotrexate studies for 12 months. These studies enrolled patients who had received previous DMARDs, as well as those who were DMARD-naive. The Phase II trial was a dose-ranging study in which subjects received placebo or leflunomide 5 mg, 10 mg, or 25 mg daily; a single loading dose was given, 50 mg for the 5 mg group and 100 mg for the 10 and 25 mg groups. There was a clear clinical dose response, with the 25 mg dose being superior to 10 mg and the 5 mg dose being ineffective.6

In the Phase III trials, a loading dose of leflunomide 100 mg taken on each of the first three days was used, followed by a maintenance dose of 20 mg daily. The dose of sulfasalazine was 1 g twice daily, while methotrexate dosing was initially 7.5 mg/week, titrated up to 15 mg/week, and 53%–60% of patients took 15 mg/week from week 9. These doses of methotrexate were those most commonly used at the time, although lower than in current practice. In the placebo-controlled methotrexate study, folic acid supplements of 1–2 mg daily were given per protocol and taken by 98% of participants, but in the active comparator trial, folic acid supplementation was optional and used by only 11%, usually after an adverse event had occurred. In contrast with other reports,27,28 adverse events due to methotrexate were no more likely in the patients not taking folic acid supplements. However, there was some evidence that folic acid reduced the efficacy of methotrexate. The tender joint count, swollen joint count, and both the physician and the patient global assessments were significantly more improved by methotrexate than by leflunomide in the study without folic acid given per protocol, whereas in the study where folic acid supplementation was mandatory, leflunomide showed greater improvements in these outcomes. Several reports and reviews have concluded that higher doses of methotrexate are required to achieve a given clinical outcome in the presence of folate supplementation,28–30 so this discrepancy, combined with the rather low doses of methotrexate used, suggests that there was some bias in these trials in favor of leflunomide.

The primary outcome measure in these studies was the American College of Rheumatology (ACR) 20 response at study endpoint; ACR 50 and ACR 70 were also recorded. Summarized results of the studies are shown in Figure 1. The mean time to initial response (ACR 20) was about eight weeks, and maximal responses were seen by 12 weeks,25 although DAS28 (Disease Activity Score including a 28-joint count) clinical responses continued to increase over the first six months of therapy in a sulfasalazine trial (Figure 2).31 Improvement in all clinical outcome measures (tender and swollen joint counts, patient and physician global assessments) occurred earlier with leflunomide than with sulfasalazine. Quality of life as assessed by the Health Assessment Questionnaire Disability Index (HAQ DI) was significantly improved by leflunomide as compared with placebo and to a similar degree as methotrexate (improvement in HAQ DI of 0.29 for leflunomide and 0.26 for methotrexate).26 Analysis of results for other quality of life measures, ie, the Medical Outcomes Survey Short Form-36 (SF-36) and the weighted top five problem elicitation technique, suggested that leflunomide was superior to methotrexate for these outcomes.25,26,32

Figure 1

Range of American College of Rheumatology responder rates from different clinical studies of leflunomide. Data at six months and one year are taken from double-blind randomized placebo and active comparator-controlled clinical trials.8,24,25 Patients completing 12 months were re-enrolled to a year 2 cohort and remained blinded to treatment allocation.33–35 Those completing two years were eligible to enroll in an open-label, non-controlled extension study to complete five years of treatment.37

Figure 2

Sustained DAS28 response over time for leflunomide 20 mg daily following a loading dose of 100 mg daily for three days (percentage of patients with a response maintained to 24 weeks). Data from the RELIEF study.51

Abbreviation: DAS 28, Disease Activity Score 28 joint count.

Long-term studies

Long-term follow-up extension studies of the monotherapy trials confirmed durability of effects in patients who had responded to initial treatment.33,34 Clinical ACR responses, radiographic progression, and quality of life indices all showed sustained improvement. Eighty-five percent of leflunomide-treated patients and 79% of the methotrexate-treated patients remained on active treatment for two years.33 In the extension studies, the dose of methotrexate could be escalated to 20 mg/week if clinically indicated. No difference was seen in maintenance of clinical improvement between leflunomide and methotrexate, but responses to sulfasalazine waned. There was an emergent difference compared with methotrexate in the second year in favor of leflunomide in quality of life and physical function indices between the active comparator drugs. In a two-year follow-up of the sulfasalazine trial, HAQ DI fell by a mean 0.65 in the leflunomide arm compared with 0.36 for sulfasalazine and no change for placebo.35 Study participants receiving leflunomide showed improvement in SF-36 scores that approached US population norms over 12 and 24 months (Figure 3).25,26,33 Radiographic analysis showed that patients responsive to leflunomide in the first year experienced a halting of disease progression in the second year.36

Figure 3

Quality of life changes in randomized controlled trials of LEF versus MTX over two years assessed by Medical Outcomes Survey Short Form 36 (SF-36). Vertical bars show baseline and 24-month data for each domain of the SF-36, dashed horizontal lines show US population norms.143

Abbreviations: LEF, leflunomide, MTX, methotrexate.

An open-label extension study of 214 patients still responding at two years in two Phase III trials was conducted until leflunomide was marketed, with a mean treatment duration of 4.6 years.37 Efficacy results by ACR responses showed that improvement seen at one year was maintained for up to five years (ACR 20 responses remained around 70%, ACR 50 at 50% and ACR 70 at 20%. [See Figure 1]). Functional ability and quality of life improvements were also maintained, and there were no new treatment-emergent side effects.

Remission

Remission is an increasingly realistic goal in RA, and has been defined by the US Food and Drug Administration (FDA),38 the ACR,39,40 and the European League Against Rheumatism (EULAR).41 Using data from clinical trials, remission rates according to these definitions have been calculated in post hoc analyses.42 Methotrexate, gold, penicillamine, and sulfasalazine showed remission rates of between 7% and 22%, while leflunomide showed EULAR remission rates between 13% and 20%.31,43 Combination therapies can show remission rates of up to 42%, and biologic drugs in combination with methotrexate 31%–50%.

Effect on structural joint damage

Radiographic arms of the studies assessed erosions by Sharp score and demonstrated a slowing of disease progression by leflunomide, sulfasalazine, and methotrexate compared with placebo.8,36,44 While improvements were shown for the comparison with placebo, treatment responses were not different between the active comparators in these Phase III registration trials. A long-term analysis of 128 patients treated for a mean of 4.3 years showed that one-third of patients treated with leflunomide had no radiographic progression.45 In a study primarily designed to show the validity of digital X-ray radiogrammetry and computer-aided joint space analysis as diagnostic tools to quantify radiographic changes occurring over time in RA, 40 patients taking either methotrexate or leflunomide were studied.46 The study was not randomized, but the patient characteristics were well matched between the treatment groups. Patients taking methotrexate 15 mg weekly showed more joint space loss and demineralization than those taking leflunomide 20 mg daily. These results suggest a strong effect on halting of joint damage by leflunomide, to at least the same extent as methotrexate.

Summary of registration studies

The registration studies for leflunomide in RA are important for several reasons. They are the last placebo-controlled studies that will ever be done in patients with active RA. They were the first well designed studies to show conclusively that DMARDs are disease-modifying in terms of radiographic progression. The trials enrolled high proportions of patients with early disease (40%–45% had disease duration of between six months and two years) and of patients who were DMARD-naive (33%–47%). The placebo arms of the trials also give a benchmark for radiographic progression in early and established RA (other studies have used cross-sectional data to demonstrate erosive progression). Leflunomide was approved in 1999, a golden year in rheumatology that also saw the approval of the anti-TNF agents, infliximab and etanercept.

Leflunomide in combination with nonbiologic DMARDS

Methotrexate

Methotrexate and leflunomide have different but complementary effects on the inflammatory immune pathway. Methotrexate inhibits cytokine production and purine synthesis and causes the release of adenosine, whereas leflunomide affects de novo pyrimidine synthesis, thereby regulating lymphocyte proliferation. This provides a biochemical basis for combination therapy in RA.47 Combination treatment using methotrexate and leflunomide has been assessed in two Phase III trials. Patients with an inadequate response to methotrexate had leflunomide added to their treatment.

In a small open-label study of 30 patients48 leflunomide 10 mg daily was added to methotrexate without a loading dose; 53% of patients achieved ACR 20 and 38% achieved ACR 50 at 12 months. No pharmacokinetic interactions were noted. In a larger, double-blind, placebo-controlled study of 263 patients with active disease despite methotrexate, dosed at 15–20 mg/week for at least six months, the combination of methotrexate with leflunomide 100 mg for two days followed by 10 mg daily was studied. The addition of leflunomide was superior to methotrexate plus placebo; at 24 weeks the ACR 20 response rate was 46.2% versus 19.5%; and ACR 50 response rate 26.2% versus 6.0% for placebo (Figure 4).49 Health-related quality of life measures and physical functioning were improved significantly by combination treatment but not by methotrexate-placebo. Discontinuation rates and side effects did not appear to be increased in the combination therapy treatment group.

Figure 4

American College of Rheumatology response rates for combination therapy of leflunomide plus methotrexate versus methotrexate plus placebo.49

Abbreviations: MTX, methotrexate; PBO, placebo; LEF, leflunomide.

A 24-week, open-label extension study enrolled 192 patients in which those receiving methotrexate-placebo were switched to methotrexate-leflunomide 10 mg daily while those already on the combination continued their treatment.50 In contrast with the original leflunomide cohort, a loading dose was not given. This enabled the clinical response and side effect profile of use of a loading dose to be examined. ACR response rates were maintained by those remaining on combination therapy, while those switching from placebo-methotrexate showed improvements in clinical outcomes and health-related quality of life similar to the original cohort. Therefore, there is a lack of evidence that using a loading dose of leflunomide improves clinical responses at 24 weeks when adding the drug to methotrexate.

Sulfasalazine

In the RELIEF study, leflunomide was studied in combination with sulphasalazine.31,51 Following 24 weeks of open-label leflunomide, nonresponders were randomized to sulfasalazine monotherapy or to sulfasalazine-leflunomide in a 24-week, double-blind, and placebo-controlled phase. The study did not show a difference between the treatment groups, but was underpowered because of a higher than predicted responder rate in the initial phase of the trial. At six months, 70% achieved a DAS28 response, 61% had an ACR 20 response, 25% had a low DAS (<3.2), and 13% were in remission,31 confirming the results from other Phase III trials. The time course of the ACR 20 response is shown in Figure 2. In the sulfasalazine-leflunomide versus sulfasalazine part of the trial, a trend towards better DAS outcome with the combination was observed but this was not statistically significant. The leflunomide-sulfasalazine combination appeared to be as well tolerated as monotherapy.

Leflunomide in combination with biologic DMARDs

Anti-TNF agents

Methotrexate is usually coprescribed with anti-TNF agents and has been shown to improve clinical response rates in RA, a practice supported by several randomized controlled trials using etanercept, infliximab, or adalimumab52–55 and supported by a large population-based study.56 Retention on therapy is also higher for methotrexate-anti-TNF than for anti-TNF monotherapy.57,58 This combination has a side effect profile similar to anti-TNF agents used alone. However, enhanced clinical responses have only been shown in RA; methotrexate is not usually coprescribed with anti-TNF agents being used for psoriasis, Crohn’s disease, or ankylosing spondylitis, even though methotrexate is used as primary therapy for most of these indications.

In patients intolerant or nonresponsive to methotrexate, other DMARDs have been used to increase the efficacy of anti-TNF agents, despite a relative lack of evidence for efficacy from prospective, randomized, and blinded trials. Two randomized controlled trials studied the effect of adding etanercept to sulfasalazine compared with continuing either treatment alone in patients with active disease despite sulfasalazine.59,60 Clinical outcomes were equal whether etanercept was used as monotherapy or in combination with sulfasalazine, and both were superior to continued sulfasalazine monotherapy. Likewise, clinical outcomes were equivalent between a range of conventional DMARDs, including leflunomide used as cotherapy with adalimumab.61,62

Observational studies

After methotrexate, leflunomide is the most commonly prescribed cotherapy with anti-TNF agents.63 Leflunomide has been used in a number of open-label studies of varying quality, and reports from registries confirm this use in practice.58,63–73 In these observational studies, an anti-TNF agent was added to leflunomide following stabilization of treatment and an inadequate response. The general conclusion has been that the combination is generally safe, and methotrexate and leflunomide appear to have equivalent efficacy in combination with anti-TNF agents. However, few studies have addressed the issue of relative efficacy in a formal analysis.

Some nonrandomized retrospective studies have suggested that leflunomide is less effective and associated with more side effects compared with methotrexate used in combination with infliximab. One prospective study suggested an increased incidence of serious adverse events, in particular severe skin reactions and immune-mediated side effects, including appearance of antibodies and severe infections, and a lower effectiveness compared with methotrexate.62 Patients given leflunomide are usually intolerant or nonresponsive to methotrexate and may be at a higher baseline risk of developing side effects (a channeling effect) and to have poorer responses to treatment, so conclusions are hard to draw.

A prospectively enrolled Swiss registry study compared the retention rate, effectiveness and safety of leflunomide with other conventional DMARDs, including methotrexate, as cotherapy with anti-TNF agents in 1218 patients with RA.74 Hazard analysis was used to analyze discontinuation rates and the incidence of toxicities as indicators of effectiveness, and longitudinal regression modeling was used to analyze radiographic progression, disability, and disease activity scores. There was no difference in retention on therapy between different groups. The discontinuation rate was high, with patients remaining on the combinations for a mean of only 16 months. This rate is substantially higher than that of anti-TNF agents used as monotherapy. Apparent differences in retention rates in favor of methotrexate disappeared when confounding variables were accounted for. There were no significant differences in response to treatment among the groups as assessed by radiographic progression, change in functional disability score, or RA disease activity scores. However, all groups improved over baseline. Twenty-eight percent of patients using combination therapies had treatment stopped because of adverse events, principally due to allergy, infection, rash, and gastrointestinal intolerance. Hepatotoxicity was an uncommon reason for discontinuation. There were no differences in tolerability or safety profile between the treatment groups, apart from a lower reported rate of allergy in the methotrexate-anti-TNF group. The authors concluded that they could detect no difference in drug retention rates or effectiveness in patients using anti-TNF agents with methotrexate, leflunomide, or other nonbiologic DMARDs.

A similar analysis was performed by a German group reporting on the biologics registry, RABBIT, in which responses to individual anti-TNF agents was examined.75 Disease activity and treatment data were analyzed from 1769 patients treated with adalimumab, etanercept, or infliximab in combination with either methotrexate or leflunomide over 36 months. Discontinuation rates at 36 months for anti-TNF-methotrexate combinations were 46.3% for etanercept, 51.3% for adalimumab, and 61.5% for infliximab. For anti-TNF-leflunomide, discontinuation rates were 53.4% for etanercept, 63.1% for adalimumab, and 67.1% for infliximab. However, patients treated with leflunomide combinations had a higher baseline disease activity score. This was highest in the leflunomide-infliximab group, which also had the highest discontinuation rate. EULAR response rates at 24 months were 74%–81% for methotrexate and 72%–81% for leflunomide combinations. The results support the use of leflunomide in combination with anti-TNF agents where methotrexate is contraindicated, not tolerated, or lacks efficacy.

Randomized study

A recent prospective randomized study involving 120 subjects compared methotrexate or leflunomide in combination with adalimumab, infliximab, or etanercept.76 Patients with high disease activity despite treatment with either methotrexate or leflunomide were followed for 24 weeks and assessed by ACR 20, 50, and 70 responses and by DAS28-ESR (erythrocyte sedimentation rate). There were no efficacy differences between the leflunomide or methotrexate combination groups or any of the six treatment subgroups. Quality of life improvements assessed by modified HAQ exceeded the minimum clinically important difference77 in all treatment allocation groups at equal time points (Figure 5). Mild side effects occurred more commonly with methotrexate (43.3%, mostly nausea) than with leflunomide (21.6%, mostly hypertension, weight loss, and diarrhea). Sixteen patients discontinued because of serious side effects. There was no difference overall between the groups in rates of discontinuation, and although more patients discontinued due to serious side effects with the leflunomide combinations, this was not statistically significant. It appears that there is a similar probability of achieving clinical improvement between methotrexate and leflunomide when used in combination with anti-TNF agents.

Figure 5

Change in quality of life occurring over time by treatment allocation with biologic DMARDs in combination with methotrexate or leflunomide.76

Abbreviations: ADA, adalimumab; ETN, etanercept; INF, infliximab; LEF, leflunomide; MTX, methotrexate; MCID, minimum clinically important difference;77 mHAQ, modified Health Assessment Questionnaire; DMARDs, disease-modifying antirheumatic drugs.

Other biologic DMARDs

Leflunomide is starting to be used in place of methotrexate in combination with other biologic DMARDs, such as rituximab, and appeared to be effective in a small case series.78 A larger multicenter study of 1901 patients compared rituximab alone or in combination with either methotrexate or leflunomide over one year. Despite patients in the combination treatment groups having higher baseline DAS28 values, significantly more patients treated with rituximab-leflunomide achieved a EULAR “good response” at six months (33%) than those treated with rituximab-methotrexate (21%) or rituximab alone (20%).79 Leflunomide has also been used with anakinra,80,81 but the effect of leflunomide in combination with other biologic DMARDs, such as tocilizumab or abatacept, has not been formally assessed.

Clinical studies in psoriatic arthritis and other inflammatory conditions

Psoriatic arthritis

In comparison with the published data on leflunomide in RA, there is a paucity of research in psoriatic arthritis, with only one 24-week Phase III trial in 190 subjects with active psoriatic arthritis and psoriasis.82 Study participants had at least 3% of the skin surface involved with psoriasis. After randomization, they received placebo or a 100 mg per day loading dose of leflunomide for three days followed by 20 mg daily. Leflunomide showed good efficacy for psoriatic arthritis and also showed a significant benefit for psoriasis. Fifty-eight of 95 participants taking leflunomide achieved the primary outcome of a psoriatic arthritis ACR response (PS-ACR) (a composite outcome measure comprising clinical, biochemical, and function components validated for psoriatic arthritis) compared with 27 of 91 taking placebo (58.9% versus 29.7%). Psoriasis was also improved83 as assessed by Psoriasis Activity and Severity Index (PASI) score, target lesion response, SF-36, and the Dermatology Life Quality Index (DLQI). A 50% improvement in PASI score (PASI 50) was achieved by 30.4% on leflunomide versus 18.9% on placebo. Significant improvements with lower response rates were also seen for PASI 75 and PASI 90 scores; target lesion response was 46.4% versus 25.3%, and quality of life improvements were significantly greater with active treatment. These results indicate a moderate degree of improvement of psoriasis with leflunomide.

A small retrospective study compared duration of treatment with leflunomide and methotrexate in 44 patients with psoriatic arthritis in a rheumatology clinic.84 Loading doses were not given, and the doses were leflunomide 10–20 mg daily and methotrexate 7.5–15 mg/week. At 24 months, 54.7% and 59% of patients, respectively, remained on the drug. There was a higher crude rate of adverse events in leflunomide users (38.7 per 100 patient-years) compared with methotrexate (14.3 events per 100 patient-years) and there was a trend towards more discontinuation for lack of efficacy with methotrexate (28.6% versus 12.6%). Despite this relative lack of evidence, leflunomide is routinely used for psoriatic arthritis and other forms of inflammatory polyarthritis.

Other inflammatory conditions

Leflunomide has also shown benefit in a small open-label study in juvenile idiopathic arthritis85 and in a larger, randomized, controlled trial comparing it with methotrexate.86 Dosing was by body weight, with those over 40 kg receiving the full adult dose of 20 mg daily and a 3 × 100 mg loading dose. For the primary outcome measure (a pediatric ACR 30 response) both treatments had a high response rate, but methotrexate was superior (89% versus 68%, P = 0.02). Leflunomide has also been investigated in small studies of systemic lupus erythematosus,87,88 Sjogren’s syndrome,89 ankylosing spondylitis,90,91 dermatomyositis,92 and for treatment93 and remission maintenance94 in Wegener’s granulomatosis. The results have been variable, and dosing and side effects do not differ among these indications.

Safety and tolerability

There is a large database on clinical safety issues with leflunomide. As with other DMARDs, side effects are fairly common, but most are mild and can be managed without discontinuation (Table 1). Side effects are most likely to occur early in treatment and do not appear to be more or less likely when leflunomide is used in combination with other DMARDs. The most common side effects are diarrhea, itchy maculopapular skin rash, reversible alopecia, and transient rises in liver enzyme test results. In Phase III trials, most side effects were mild to moderate, and occurred in the first six months of therapy, with a tendency for problems to diminish over time.37 More significant health problems related to leflunomide use are rarer, and include hypertension, bone marrow suppression, peripheral axonal neuropathy, interstitial pneumonitis, and teratogenicity.

Table 1

Management strategies for side effects of leflunomide

	Diarrhea	Alopecia	Liver enzymes	Hypertension	Skin rash	Cytopenia
Occurrence	24% in trials, withdrawals 2.2%.12	14% in trials, withdrawals 1%.Usually mild and transient.	5%–10% had rises in transaminases. Most common in first 6 months. No cumulative effect or cirrhosis reported.	Occurs in up to 10% usually in known hypertensives or with NSAID use.New onset hypertension 1.6%, equal to placebo in trials. Sudden rise in BP has been reported.	12% in trials, discontinuation 1%.12 SJS and TEN occur.	These occur but are rare with monotherapy.
Prediction	Patients with loose bowel motions more likely to be affected.	No predisposing factors identified. Ask patient how acceptable hair fall would be.	Avoid in patients with pre-existing liver disease.Caution with patients who have had liver toxicity with other drugs.	Measure blood pressure at baseline. Record history of hypertension and treatment.Record all drugs that may elevate blood pressure.	Ask about allergy, in particular any past cutaneous reactions to drugs.	Baseline CBC to assess significance of any fall on treatment.
Prevention	Avoid loading dose, start at 10 mg/day or 10 mg alternate days.	Avoid loading dose.	Screen for hepatitis B and C, pretreatment liver enzymes. Monitor patients on therapy. Advise against alcohol use.	Monitor blood pressure while on treatment. Avoid use in uncontrolled hypertension.	Caution in patients with prior history of cutaneous reactions to drugs	CBC every 2–4 weeks for first 3 months, every 8–12 weeks thereafter.Caution in combination with methotrexate or if there is a history of drug induced cytopenia
Management	Mild/moderate: reduce dose or frequency. Moderate: use antidiarrheal agent short term. Severe: stop drug for 1–2 weeks	Reduce dose or frequency.Discontinue if severe.	For persistent transaminase rise <2 × ULN reduce dose.Discontinue treatment and consider washout for more significant rises.98	Mild rise (SBP > 140 < 170 mmHg or DBP > 90 mmHg <110 mmHg): reduce dose. Very high BP (SBP > 170 mmHg, DBP > 110 mmHg): discontinue	Mild: continue and use antihistaminesModerate: stop temporarilySevere: discontinue and give washout (anaphylaxis, bullous reactions, purpura, mucosal lesions, fever)	Evaluate for possible causes. Look for signs of infection.Mild changes: observe or reduce dose. Moderate falls: temporarily withhold treatment. Severe cases (neutrophil count <1.0 × 109/L) discontinue and use washout.
Education	Tell patients about risk of diarrhea, manage expectations.	Discuss with patient sensitively. Emphasize reversibility of condition.	Discuss alcohol use. Emphasize importance of regular blood test monitoring.	Advise patients of the need to have blood pressure checked while on treatment (eg, at GP or nurse clinic).	Tell patients about risk of rashes.

Abbreviations: BP, blood pressure; SBP, systolic blood pressure; DBP, diastolic blood pressure; SJS, Stevens Johnson Syndrome; TEN, toxic epidermal necrolysis; CBC, complete blood count; GP, general practitioner.

Hepatotoxicity

Since its launch, there has been a steady stream of reports regarding fatal liver injury in association with leflunomide. A nested case-control study of 41,885 patients with RA dispensed a DMARD from two different claims databases concluded that leflunomide was no more likely to be associated with serious liver toxicity than methotrexate, and was less likely to cause hepatic injury than biologic DMARDs.95 The study did not find any increased association of nonserious hepatic events with leflunomide as compared with other DMARDs. An observational study of 101 RA patients treated with leflunomide for a mean of 10 (range 0.5–12) months found an incidence of 9% for a rise in transaminases of 2–3 times the upper limit of the normal range (2–3× ULN).96 Reporting from the CORRUNA database, a recent study found an increased rate of liver enzyme rises when leflunomide is used with methotrexate, but not for leflunomide monotherapy compared with methotrexate.97 There were 1953 patients with RA and 151 with psoriatic arthritis in the study. In RA, rises in alanine aminotransferase (ALT) or aspartate aminotransferase (AST) of 1–2× ULN occurred in 14%–22% of patients treated with methotrexate, leflunomide, or other DMARD monotherapy, but occurred in 31% of those on a methotrexate-leflunomide combination. Rises of >2× ULN occurred in 5% of these patients. Analysis showed this increased risk was related to the dose of methotrexate (10–17.5 mg/week compared with >20 mg/week). Raised liver transaminases occurred in 35% of patients using methotrexate for psoriatic arthritis, confirming previous observations that liver toxicity is more common in psoriasis, but there were insufficient patients with psoriatic arthritis treated with leflunomide for analysis.

In 2010, the FDA placed a “black boxed” warning for liver failure on the leflunomide datasheet.98 The committee reviewed 49 cases of severe liver injury between 2002 and 2009. There were 14 deaths, five patients who received a liver transplant, and a further nine who had a life-threatening episode. Jaundice, coagulopathy, or encephalopathy was described in another 11 cases, and the remaining patients had other milder manifestations of liver toxicity, such as rash, itch, vomiting, abdominal pain, and fever. Seventeen of the 49 patients had normal liver enzymes before starting leflunomide. In 46 of the 49 cases, patients were also taking drugs with known liver toxicity, including methotrexate, anti-TNF agents, hydroxychloroquine, NSAIDs, or acetaminophen. The FDA advises that patients with ALT values > 2× ULN should not be given leflunomide, and that leflunomide should be stopped, and a washout procedure considered in any patient in whom the ALT rises above 2× ULN while on treatment. This is consistent with current consensus statements for monitoring of patients on leflunomide endorsed by the ACR, although persistence of changes should be observed before instituting washout.99 Patients should be screened for hepatitis B and C prior to starting leflunomide, and particular care should be used when using leflunomide in combination with other drugs with known hepatotoxicity.99 As with methotrexate, it is also appropriate to advise patients to drink a minimum of alcohol while using leflunomide.

Interstitial pneumonitis

The association of leflunomide with acute interstitial pneumonitis was first described in the Japanese literature, and it remains a problem that seems more common in this group. In a recent study of 5054 patients in Japan prospectively followed for the first 24 weeks of treatment, the incidence of newly developed or exacerbated interstitial lung disease in RA patients was 1.2%.100 Pre-existing interstitial lung disease, cigarette smoking, body weight less than 40 kg, and use of a loading dose emerged as independent risk associations in multivariate analysis. As with other causes of acute pneumonitis, there is a high case fatality rate. In one study, nine of 22 patients died, and profound hypoxemia, a low serum albumin, a high serum C-reactive protein level, and failure of recovery of lymphocytopenia were associated with poor outcome.101

A number of other groups have reported on leflunomide and pneumonitis, but because these are mostly retrospective case series or summaries of spontaneous adverse event reporting, there are problems of confounding and bias. Adjudication of cases also caused significant difficulty because, in many cases, the clinical presentation was not well described and other causes were not always excluded. In almost all cases, methotrexate is also implicated, although the occurrence of the problem in close temporal proximity to starting leflunomide is recognized. In a nested case-control study of 62,734 RA patients treated with DMARDs, there were 74 cases of acute pneumonitis. Compared with DMARD controls, there was a relative risk of 1.9 for developing pneumonitis when taking leflunomide.102 There was no increased risk with leflunomide when patients exposed to methotrexate or who had pre-existing interstitial lung disease were excluded. A recent review has brought some clarity.103 From a literature search, 32 cases were identified satisfying adjudication criteria, and 19% of patients died. In all of the cases, the patients had either a prior history of interstitial lung disease or had taken methotrexate and, in 44%, methotrexate was being used in combination with leflunomide. Although there was a tendency for pneumonitis to occur earlier if a loading dose was used, it did not appear that use of a loading dose increased the likelihood of this complication. Cholestyramine washout did not affect outcome. In all cases the patients had RA, and pneumonitis has not been described in patients using leflunomide for other indications. It seems likely that leflunomide, like other immunomodulatory agents, including the anti-TNF agents, increases the likelihood of pneumonitis associated with RA. Leflunomide does not seem to be associated with slowly progressive lung fibrosis or other pulmonary side effects, although there are isolated reports of pulmonary nodulosis.104,105

Hypertension

Hypertension has been commonly reported as a side effect of leflunomide. In the Phase II study it occurred in up to 10.6% of patients given the 25 mg daily dose.6 New onset of hypertension occurred in the Phase III clinical trials in 2.1%–3.7% of patients, and mean increases in systolic and diastolic blood pressures were 2.2 mmHg and 1.9 mmHg, respectively.8,25,34 These changes were not associated with renal dysfunction or proteinuria. Various mechanisms have been suggested, including displacement of NSAIDs from protein binding sites and an increase in sympathetic drive.106 In a prospective study of 30 patients treated with the standard treatment algorithm, a significant rise in systolic blood pressure from a mean of 127 mmHg to 134 mmHg occurred within 2–4 weeks.107 Mean diastolic blood pressure did not increase significantly, but in one patient a rise of 40 mmHg systolic and 20 mmHg diastolic blood pressures were noted. Therefore, it is advisable that blood pressure be monitored, particularly in the first few weeks of treatment.

Neuropathy

Central nervous system side effects reported in clinical trials include dizziness (4%), headaches (7%), and paresthesiae (2.9%). While paresthesiae were more common with leflunomide than with sulfasalazine or with placebo, the incidence was similar in patients taking methotrexate. Peripheral neuropathy, specifically a sensory axonal neuropathy, has been suggested as a side effect of leflunomide in several case reports108–111 and in a retrospective case series of 785 patients that suggested a prevalence of 1.4%.112 It has occurred in patients treated for RA and psoriatic arthritis. In a review of 80 cases reported to the FDA, symptoms developed most commonly within six months of starting leflunomide (range three days to three years). Nerve conduction studies in 37 cases showed a distal axonal sensory or sensorimotor polyneuropathy, and recovery was usual on stopping the drug.113 A prospective observational study of 113 patients with RA treated with leflunomide found eight incident cases and two cases of exacerbated peripheral neuropathy.114 Thirty percent of cases had concomitant diabetes compared with 2.9% for those without neuropathy, and potentially neurotoxic drugs were in use by another 20% versus 1.9%. In a prospective cohort study of 16 patients treated with leflunomide and 32 RA patients treated with other DMARDs followed for six months, peripheral neurologic symptoms were specifically sought, and all patients underwent nerve conduction studies. An increase in neurologic symptoms was reported in 54% of the leflunomide group versus 8% of the control group.115 However, there was no correlation with the results of neuroelectrophysiologic studies. It can be concluded that paresthesiae are a fairly common side effect of leflunomide therapy, and that peripheral neuropathy remains a rare event with an uncertain causal relationship with leflunomide.

Weight loss, diabetes

Weight loss was reported in the original Phase II study, but has not been confirmed subsequently in trials.6 An observational study found that 7% of patients treated with leflunomide experienced significant weight loss (8–20 kg) that could not be explained.116 Cachexia sometimes occurs by an unknown mechanism. It does not correlate with diarrhea or nausea. Because DHODH is a mitochondrial enzyme, it is possible that leflunomide causes an increased metabolic requirement by uncoupling of oxidative phosphorylation, reducing the production of adenosine triphosphate. Weight loss is described as a common side effect in the data sheet.5

Diabetes is mentioned in the data sheet for leflunomide as occurring in 1%–3% of patients taking leflunomide in clinical trials,5 but published reports detailing this putative side effect are hard to find. Leflunomide has been used in experimental models of autoimmune diabetes and appears to be protective. Patients may ask about this possible complication, which does not seem to be a clinically significant problem.

Bone marrow suppression

Cytopenia is rare with leflunomide but bone marrow suppression has been reported, usually in association with other known causes, such as concomitant drug use and illness. There have been case reports of respiratory tract infections in patients taking leflunomide117–120 and of classical pulmonary tuberculosis121,122 but there are no reports of reactivation of latent tuberculosis. There may be an increase in susceptibility to infections,123–125 but there is no robust evidence to suggest these occur more frequently with leflunomide than in the general population of people with RA treated with nonbiologic DMARDs.

Skin rash and wound healing

Nonspecific itchy skin rash occurs with leflunomide and appears more likely in people with prior skin reactions to drugs. The incidence was 7.4%–24% compared with placebo rates of 4%–14% in trials.7,19,20 More serious skin reactions, including Stevens-Johnson syndrome and toxic epidermal necrolysis, have been reported rarely.5,126

There is uncertainty regarding the effect of leflunomide on wound healing and postoperative complications, because there are only a few conflicting reports.127 One observational study of 201 patients undergoing orthopedic procedures suggested that there were more postoperative complications and poor wound healing in patients treated with leflunomide.128 The study was nonrandomized and not blinded, and included a wide range of underlying conditions and treatment combinations. Another prospective randomized study in 82 patients showed no increased risk if leflunomide was continued rather than stopped prior to surgery.129 Therefore, it would be premature to conclude that leflunomide should be discontinued routinely for patients undergoing surgery.

Use of leflunomide in clinical practice

Retention on leflunomide

The length of time a patient stays on a DMARD is an accepted clinical indicator of effectiveness, although it is influenced by a number of factors that introduce bias.130 In the six-month and 12-month Phase III trials, retention on leflunomide ranged from 17% to 44%, but annualized rates (eg, per 100 patient-years) were not given.8,24,26,44 In the extension studies, retention rates were higher (82%–85%) but patients were re-enrolled into the studies, so those patients who were intolerant or partially responsive may have chosen not to continue.33,34 Because the data were prospectively gathered in randomized patient groups, there is some reassurance regarding tolerability with long-term use. Responses in clinical trials are not usually replicated in real-life clinical situations, so information from postmarketing studies is very relevant.

There have been several postmarketing cohort studies that have examined the length of time patients remain on leflunomide treatment,96,131–137 all of which have found higher rates of discontinuation than those observed in clinical trials. In a multicenter case series of 136 patients followed for a median 317 days, the discontinuation rate was 56.2 per 100 patient-years.136 Twenty-nine percent of patients stopped for an adverse event and 13% because of lack of efficacy. In a subsequent study, this group reported on 279 patients started on leflunomide in whom 62% discontinued in the treatment period (annualized discontinuation rate was not given). Using multivariate analysis, a strong effect was seen for “attending rheumatologist”, suggesting that physician behavior in response to side effects is an important determinant.135

A study in France followed 116 patients over three years. The discontinuation rate was 70% at one year, being 32% because of an adverse event and 22% for lack of efficacy.133 An observational retrospective study using the US Veterans Affairs database, which involved 3325 predominantly male patients over 33 months, reported a discontinuation rate of 42% and found an association with use of a loading dose.134

In another observational study in two centers, leflunomide was compared with sulfasalazine and methotrexate in 1088 patients, comprising 5141 patient-years of DMARD exposure.132 Time to discontinuation was studied using Kaplan–Meier analysis, and showed that duration of treatment was longer for methotrexate (median 28 months versus 20 months for leflunomide or sulfasalazine). The overall discontinuation rate was 55% after two years of follow-up, and another retrospective postmarketing study found a withdrawal rate of 26% at 30 months.131

In a national postmarketing surveillance study in New Zealand, 318 patients were prospectively enrolled and followed for two years.137 The discontinuation rate was 36% at one year and 50% at two years. Overall, these observational studies show that leflunomide is an effective treatment in the clinic, with about 50% of patients remaining on treatment long term.

Leflunomide in the elderly and in early RA

Leflunomide is well tolerated and effective in elderly people with RA and psoriatic arthritis.138 In a retrospective study of 90 people (10 with psoriatic arthritis), 50 of whom were aged over 65 years, discontinuation rates at 24 months were 34% in those under 65 and 32.5% in the older group. There were no differences based on leflunomide being used as monotherapy or in combination with other DMARDs, and there was no difference in the occurrence of or withdrawals due to adverse events.

The effectiveness of leflunomide in early RA was suggested by analyses of the Phase III clinical trials and confirmed in a recent prospective open-label study of people with disease duration less than one year.139 A DAS response was achieved in 71.9% at 12 weeks and 84.6% at 24 weeks, and 25% achieved remission by DAS28 criteria. Reported side effects were less frequent than in other studies in established disease (1%–3% experienced diarrhea, nausea, hypertension, or headache). These observations confirm previous observations that DMARDs are generally more effective and better tolerated in early RA.

Use of a loading dose

In the Phase III clinical trials of leflunomide as monotherapy, a loading dose of 100 mg daily for three days was given, followed by a daily dose of 20 mg. Although this regimen results in a faster clinical response, the practice is widely believed to be associated with an increase in side effects, particularly liver enzyme rises, diarrhea, and hair fall. Attempting to avoid these problems, clinicians have used a variety of different loading regimes, but none has been formally studied in prospective randomized trials. Commonly, 100 mg is dosed once weekly for three weeks, with a 20 mg daily maintenance dose being used from the start, while many clinicians choose to use no loading dose at all.

The effect of a loading dose on tolerability was examined in an open-label extension to the Phase III trial of the combination with methotrexate described earlier in the text.49,50 Patients in the initial cohort received leflunomide 10 mg daily after a loading dose, but those inadequately controlled on methotrexate-placebo were switched at 24 weeks to methotrexate-leflunomide without a loading dose. Side effects of nausea, diarrhea, and raised liver transaminases occurred less often in the group not given a loading dose.50

In clinical practice it is now common not to use loading doses, but because this results in steady state of blood levels of leflunomide not being achieved for up to two months5 owing to its very long half-life, loading doses should probably be considered more often. The observation that clinical responses may not be maximal for up to six months, even with use of a loading dose,51 suggests that a three-month trial of therapy may be insufficient. A registry study using hazard analysis found no effect of the use of a loading dose on retention on treatment with leflunomide,137 suggesting that the side effects, although more frequent, were easy to manage and acceptable to patients and prescribers in order to achieve a faster onset of action.

Methotrexate and leflunomide can also be started together; 72% achieved an ACR 20 at 20 weeks in one study.140 The usual dose for leflunomide in combination with methotrexate was 10 mg in these studies; 39% of patients achieve an adequate clinical response.49 Depending on patient factors, such as tolerability, methotrexate dosing can either be maintained or reduced to 10 mg weekly on starting leflunomide. However, the complexities of dosing in clinical practice have not been formally tested in a trial.

Starting dose of leflunomide: 20 mg versus 10 mg

Initial dose-ranging studies used leflunomide 5 mg, 10 mg and 25 mg daily; 20 mg was determined as the optimum dose from modeling of the clinical response.5 Since then, only 20 mg and 10 mg daily have been used in trials. In one trial, a noninferiority design was used in 404 patients with RA. Following use of a loading dose, subjects received either 10 mg or 20 mg leflunomide daily.141 ACR response rates, HAQ DI, and individual clinical measures other than the swollen joint count were more improved by 20 mg than by 10 mg daily. Patients taking the lower dose also used a higher mean dosage of prednisolone and experienced more side effects and withdrawals for lack of efficacy. Although some patients will gain good control using the 10 mg dose, more will gain benefit only at 20 mg. A dose of 5 mg daily is not effective. The data support using an initial dose of 20 mg daily in all patients on monotherapy, with the dose reduced to 10 mg daily or 10 mg alternated with 20 mg daily if needed in order to improve tolerability. The effectiveness of these strategies has not been formally tested.

Management of side effects

Hepatotoxicity

Liver enzyme changes (principally AST and ALT) may occur acutely or show a slower rise over time, and are often transient, resolving without dose adjustment of leflunomide. Alkaline phosphatase and gamma glutamyl transferase may also rise, but bilirubin is not usually affected. Changes in the ALT are of most concern. Rises that are up to 2× ULN do not necessarily require action but if the changes are persistent or if the ALT is >2× ULN, leflunomide dose should be reduced or leflunomide may need to be stopped. The FDA Drug Safety Committee recommends using a washout procedure under these circumstances98 but, because most liver enzyme changes are transient and a washout procedure can result in a flare of rheumatoid disease activity that disadvantages the patient, this management decision requires careful consideration of the balance between safety and efficacy for the individual.

Cytopenia

Cytopenia (principally neutropenia) usually occurs gradually over time and can also resolve spontaneously or with dose reduction. Unlike sulfasalazine, leflunomide has not been associated with agranulocytosis. Cytopenia is uncommon with monotherapy and is more usually associated with combination therapy, especially with methotrexate. The issue for practice is which drug to reduce or stop in this situation, but there are no trials to inform this decision. Frequently it is the combination that causes the problem, individual drugs dosed separately being well tolerated. Dose changes with methotrexate are easier to make, but leflunomide levels fall slowly after dose reduction or stopping treatment. Because leflunomide is usually added to methotrexate, it is usual to adjust the leflunomide dose in this situation, but if methotrexate has only been partially effective it may be more logical to switch to leflunomide as monotherapy.

Hypertension

It is important to be aware of the association of leflunomide with hypertension. In clinical trials, 1.6% of patients had new onset of hypertension, a rate similar to placebo. However, the overall incidence of hypertension as a side effect is closer to 10%. No studies have been done on the effect of dose reduction on blood pressure, but it is usually necessary to reduce dose or stop leflunomide, and prescribe antihypertensive agents to manage the problem. In clinical practice, leflunomide is often overlooked as a possible contributory factor to hypertension.

Other side effects

Hair fall, diarrhea, mild skin rashes, nausea, and paresthesiae may all improve with dose reduction. For some people, ongoing hair fall remains a problem necessitating cessation of therapy. However, in trials, methotrexate use is equally associated with hair fall,24 and active inflammatory disease can also cause hair loss. Diarrhea (looseness of stool) is usually mild, not associated with urgency, and is generally well accepted by patients. In some cases, however, diarrhea can be more extreme and only resolves on stopping treatment. As noted above, paresthesiae are relatively common and often respond to dose reduction. Peripheral neuropathy can be hard to ascribe to treatment and is often part of the underlying rheumatoid condition or concomitant diabetes; leflunomide should usually be reduced in dose or stopped because it may exacerbate the symptoms.

Pregnancy

Leflunomide is a known teratogen and is contraindicated in pregnancy (category X) and in breastfeeding, carrying a boxed warning in the US. Therefore, it should not be used in women wishing to become pregnant, and although the effect on male reproduction is unknown, this advice is extended to men. If leflunomide has been used within the two previous years, it is recommended that blood levels of teriflunomide be checked, and a washout procedure offered if detectable levels are found (>0.02 mg/L). In some countries it is hard to obtain blood levels and cost can be a barrier, in which case a washout procedure should be undertaken. Women who become pregnant by accident while on leflunomide should have a washout procedure and should also be counseled about the risks to fetal development.

There are many case reports of successful pregnancy outcome in this situation and so termination of pregnancy is not usually advised. A recent case-control study compared 64 pregnant women exposed to leflunomide, 95% of whom had cholestyramine washout, with 108 women with RA not treated with leflunomide and 78 healthy pregnant control subjects.142 Major structural defects were observed in three of 56 live births (5.4%) in the leflunomide-exposed women compared with 4.2% in either control group. These rates are similar to those in the general population. Therefore, there was no evidence that leflunomide exposure increases the risk of adverse pregnancy outcome in women who undergo cholestyramine washout.

Washout procedure

In any case where there is significant harm associated with leflunomide, a washout procedure should be undertaken. Protocols using activated charcoal (50 g four times daily for 11 days) or cholestyramine (8 g three times daily for 11 days) can be used; dosing does not have to be on consecutive days. A shorter regimen using cholestyramine 8 g three times daily for two days reduces plasma levels by 49%–65%.12 If this modified procedure is used, its effectiveness should be checked by measuring teriflunomide blood levels. In clinical practice, washout procedures and blood levels are done rarely,137 and there should probably be a greater use when managing severe reactions.

Screening and monitoring patients on therapy

Patients about to start leflunomide should have baseline tests similar to those for methotrexate and in practice most of these will have already been done because it is unusual to start leflunomide before methotrexate has been trialed. Complete blood count, liver enzymes, renal function, hepatitis B and C serology, HIV screen, and pregnancy test if indicated are recommended by ACR consensus guidelines at baseline.99 Full blood count and liver enzymes should be checked every 2–4 weeks in the first three months of treatment, every 8–12 weeks between three and six months, and every 12 weeks thereafter. More frequent testing is needed after an increase in dose or change in concomitant DMARD.99

Summary

Leflunomide has been in routine clinical use for RA and psoriatic arthritis for over a decade and can be considered a standard therapy. It shows efficacy and effectiveness similar to methotrexate when used as monotherapy, but its place in treatment should be after failure of methotrexate and before use of biologic DMARDs. It can be used as a combination therapy with methotrexate or sulfasalazine with enhanced efficacy where other DMARD therapy has been partially effective. It can be used in place of methotrexate in combination with anti-TNF DMARDs. In general, its side effect profile is similar to that of methotrexate, and it has a favorable risk/benefit ratio compared with other DMARDs. Postmarketing studies suggest that patients are likely to remain on leflunomide for at least as long as methotrexate. Leflunomide remains one of the most useful of the nonbiologic DMARDs for chronic inflammatory arthritis.