May S Freag1, Yosra Sr Elnaggar1,2, Doaa A Abdelmonsif3, Ossama Y Abdallah1. 1. Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt. 2. Department of Pharmaceutics, Faculty of Pharmacy & Drug Manufacturing, Pharos University in Alexandria, Alexandria, Egypt. 3. Department of Medical Biochemistry, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Abstract
AIM: This work spotlights on fabrication of CD44-tropic, layer-by-layer (LbL) coated, liquid crystalline nanoparticles of rapamycin (Rap-LbL-LCNPs) to enhance its water solubility and enable its anticancer use. METHODS: Rap-LCNPs were fabricated using hydrotrope method and then coated using LbL self-assembly technique. RESULTS: LbL coating strategy successfully reduced monoolein-induced hemolysis and increased LCNPs serum stability. Lyophilized Rap-LbL-LCNPs were successfully sterilized using γ-radiations. In CD44-overexpressed MDA-MB-231 cells, Rap-LbL-LCNPs demonstrated superior cytotoxicity compared with the nontargeted formulation. Rap-LbL-LCNPs showed 3.35-fold increase in bioavailability compared with free drug. Rap-LbL-LCNPs significantly inhibited tumor growth, enhanced animal survival and reduced nephrotoxic and hyperglycemic effects of Rap. CONCLUSION: LbL coating strategy of Rap-LCNPs could serve as a promising approach that facilitates Rap use in cancer therapy.
AIM: This work spotlights on fabrication of CD44-tropic, layer-by-layer (LbL) coated, liquid crystalline nanoparticles of rapamycin (Rap-LbL-LCNPs) to enhance its water solubility and enable its anticancer use. METHODS:Rap-LCNPs were fabricated using hydrotrope method and then coated using LbL self-assembly technique. RESULTS: LbL coating strategy successfully reduced monoolein-induced hemolysis and increased LCNPs serum stability. Lyophilized Rap-LbL-LCNPs were successfully sterilized using γ-radiations. In CD44-overexpressed MDA-MB-231 cells, Rap-LbL-LCNPs demonstrated superior cytotoxicity compared with the nontargeted formulation. Rap-LbL-LCNPs showed 3.35-fold increase in bioavailability compared with free drug. Rap-LbL-LCNPs significantly inhibited tumor growth, enhanced animal survival and reduced nephrotoxic and hyperglycemic effects of Rap. CONCLUSION: LbL coating strategy of Rap-LCNPs could serve as a promising approach that facilitates Rap use in cancer therapy.
Authors: Ahmed O Elzoghby; Shaimaa K Mostafa; Maged W Helmy; Maha A ElDemellawy; Salah A Sheweita Journal: Pharm Res Date: 2017-06-22 Impact factor: 4.200
Authors: Sarah Mokhtar; Sherine N Khattab; Kadria A Elkhodairy; Mohamed Teleb; Adnan A Bekhit; Ahmed O Elzoghby; Marwa A Sallam Journal: Front Chem Date: 2022-03-22 Impact factor: 5.221
Authors: Haidy Abbas; Nesrine S El Sayed; Nancy Abdel Hamid Abou Youssef; Passent M E Gaafar; Mohamed R Mousa; Ahmed M Fayez; Manal A Elsheikh Journal: Pharmaceutics Date: 2022-05-06 Impact factor: 6.525
Authors: Noha Khalifa Abo Aasy; Doaa Ragab; Marwa Ahmed Sallam; Doaa A Abdelmonsif; Rania G Aly; Kadria A Elkhodairy Journal: Int J Nanomedicine Date: 2019-09-17