Gabriel Alfranca1,2, Álvaro Artiga2, Grazyna Stepien3, María Moros4, Scott G Mitchell2, Jesús M de la Fuente1,2. 1. Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film & Microfabrication Technology of the Ministry of Education, Shanghai Jiao Tong University, 200240-Shanghai, PR China. 2. Instituto de Ciencia de Materiales de Aragón (ICMA-CSIC), Universidad de Zaragoza, 50009-Zaragoza, Spain. 3. Instituto de Nanociencia de Aragón, Universidad de Zaragoza, 50018-Zaragoza, Spain. 4. Istituto di Scienze Applicate e Sistemi Intelligenti 'Eduardo Caianiello,' 80078-Naples, Italy.
Abstract
AIM: This work compares the synthesis, heating capability, cellular internalization and thermoablation capacity of two different types of anisotropic gold nanoparticles: gold nanorods (NRs) and nanoprisms (NPrs). METHODS: Both particles possess surface plasmon resonance absorption bands in the near-IR, and their heating efficiency upon irradiation with a continuous near-IR laser (1064 nm) was evaluated. The cellular internalization, location and toxicity of these PEG-stabilized NPrs and NRs were then assessed in the Vero cell line by transmission electron microscopy and inductively coupled plasma mass spectrometry analysis, and their ability to induce cell death upon laser irradiation was then evaluated and compared. RESULTS & CONCLUSION: Although both nanoparticles are highly efficient photothermal converters, NRs possessed a more efficient heating capability, yet the in vitro thermoablation studies clearly demonstrated that NPrs were more effective at inducing cell death through photothermal ablation due to their greater cellular internalization.
AIM: This work compares the synthesis, heating capability, cellular internalization and thermoablation capacity of two different types of anisotropic gold nanoparticles: gold nanorods (NRs) and nanoprisms (NPrs). METHODS: Both particles possess surface plasmon resonance absorption bands in the near-IR, and their heating efficiency upon irradiation with a continuous near-IR laser (1064 nm) was evaluated. The cellular internalization, location and toxicity of these PEG-stabilized NPrs and NRs were then assessed in the Vero cell line by transmission electron microscopy and inductively coupled plasma mass spectrometry analysis, and their ability to induce cell death upon laser irradiation was then evaluated and compared. RESULTS & CONCLUSION: Although both nanoparticles are highly efficient photothermal converters, NRs possessed a more efficient heating capability, yet the in vitro thermoablation studies clearly demonstrated that NPrs were more effective at inducing cell death through photothermal ablation due to their greater cellular internalization.
Authors: Álvaro Artiga; Sonia García-Embid; Laura De Matteis; Scott G Mitchell; Jesús M de la Fuente Journal: Front Chem Date: 2018-06-22 Impact factor: 5.221