Violeta Aburto-Luna1, Samuel Treviño1, Gerardo Santos-López2, Diana Moroni-González1, Oscar Calva-Cruz1, Patricia Aguilar-Alonso1, Bertha Alicia León-Chávez1, Eduardo Brambila3,4. 1. Laboratorio de Investigaciones Químico Clínicas, Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico. 2. Laboratorio de Biología Molecular y Virología, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Hospital General de Zona No. 5, Km. 4.5 Carretera Federal Atlixco-Metepec, Metepec, Puebla, Mexico. 3. Laboratorio de Investigaciones Químico Clínicas, Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico. eduardobrambila1@yahoo.com.mx. 4. Ciudad Universitaria, 14 Sur Esq. Av. San Claudio. Edif. FCQ1, Jardines de San Manuel, 72570, Puebla, Mexico. eduardobrambila1@yahoo.com.mx.
Abstract
OBJECTIVE: To study the relationship between the release of inflammatory cytokines and mobilization of zinc into liver, and the expression of metallothionein and Zip14 transporter after an abdominal surgery in rats. MATERIALS: Thirty-five male Wistar rats were subjected to experimental surgical stress, then the subgroups of five animals were killed at 3, 6, 9, 12, 16, 20 and 24 h. Matched groups without surgery were used as controls. METHODS: Zinc levels were determined by AAS, intracellular zinc by zinquin and dithizone staining. Hepatic metallothionein was assayed by a Cd-saturation method, and IL-1β, IL-6, and TNF-β by immunoassays. Zip14 expression was analyzed by real-time RT-PCR, and protein level by immunohistochemistry and Western blot. RESULTS: Experimental surgery produced a hypozincemia, and the increase of hepatic zinc also produced the release of IL-1β, IL-6 in serum, and the increase of hepatic MT. Histochemistry showed a decrease of free zinc at 3-6 h, but an increase at 9 h (zinquin); meanwhile, total intracellular zinc increased after 9 h (dithizone). RNAm and protein levels of Zip14 were elevated between 6 and 20 h after surgery. CONCLUSION: Biochemical changes described in this work could be part of the APR, and directed to respond to the damage produced during surgical trauma.
OBJECTIVE: To study the relationship between the release of inflammatory cytokines and mobilization of zinc into liver, and the expression of metallothionein and Zip14 transporter after an abdominal surgery in rats. MATERIALS: Thirty-five male Wistar rats were subjected to experimental surgical stress, then the subgroups of five animals were killed at 3, 6, 9, 12, 16, 20 and 24 h. Matched groups without surgery were used as controls. METHODS: Zinc levels were determined by AAS, intracellular zinc by zinquin and dithizone staining. Hepatic metallothionein was assayed by a Cd-saturation method, and IL-1β, IL-6, and TNF-β by immunoassays. Zip14 expression was analyzed by real-time RT-PCR, and protein level by immunohistochemistry and Western blot. RESULTS: Experimental surgery produced a hypozincemia, and the increase of hepatic zinc also produced the release of IL-1β, IL-6 in serum, and the increase of hepatic MT. Histochemistry showed a decrease of free zinc at 3-6 h, but an increase at 9 h (zinquin); meanwhile, total intracellular zinc increased after 9 h (dithizone). RNAm and protein levels of Zip14 were elevated between 6 and 20 h after surgery. CONCLUSION: Biochemical changes described in this work could be part of the APR, and directed to respond to the damage produced during surgical trauma.
Authors: Juan P Liuzzi; Louis A Lichten; Seth Rivera; Raymond K Blanchard; Tolunay Beker Aydemir; Mitchell D Knutson; Tomas Ganz; Robert J Cousins Journal: Proc Natl Acad Sci U S A Date: 2005-04-29 Impact factor: 11.205
Authors: Erica John; Thomas C Laskow; William J Buchser; Bruce R Pitt; Per H Basse; Lisa H Butterfield; Pawel Kalinski; Michael T Lotze Journal: J Transl Med Date: 2010-11-18 Impact factor: 5.531
Authors: Kira G Slepchenko; Si Chen; Grace P Counts; Kathryn L Corbin; Robert A Colvin; Craig S Nunemaker Journal: Metallomics Date: 2021-09-02 Impact factor: 4.636