Wei-Jun Gu1, Qing-Guo Zhang1, Wei Zhu1, Yu-Lin Guo1, Lu-Qin Zhang2. 1. Department of Hematology, Rheumatology and Immunology, The Xuzhou Municiple First People's Hospital, Xuzhou 221002, Jiangsu Province,China. 2. Department of Hematology, Rheumatology and Immunology, The Xuzhou Municiple First People's Hospital, Xuzhou 221002, Jiangsu Province,China. E-mail:qq102367@163.com.
Abstract
OBJECTIVE: To investigate the imbalance of Tc1/Tc2,Th1/Th2 and Tc/Th in patients with systemic lupus erythematosus(SLE) and its relationship with the clinical stages of SLE. METHODS: The full blood culture and flow cytometry with fluorescence-labelled T lymphocytes were used to detect the levels of T-lymphocyte subsets and its intracellular factors IFN-γ and IL-4 in peripheral blood from SLP patients in active, inactive stages and normal healthy persons as controls, to compare the changes of Tc1,Tc2; Th1,Th2 levels and Tc/Th ratio in SLP patients in active and inactive stages, and to analyze their relationship with SLEDAI staging. RESULTS: Compared with healthy controls, the levels of CD4+/CD8+ in active SLE patients were significantly lower, but the levels of Tc1,Th1 in active SLE patients were higher than those in inactive SLE patients and normal controls. The ratio of Tc1/Tc2 and Th1/Th2 in the active SLE was higher than that in inactive SLE and normal controls(P<0.05), but the difference between inactive SLE patients and controls was not statistically significant (P>0.05). CONCLUSION: The Tc/Th imbalances and change of Tc and Th cells to Tc1 and Th1 cells play an important role in the pathogenesis of SLE.
OBJECTIVE: To investigate the imbalance of Tc1/Tc2,Th1/Th2 and Tc/Th in patients with systemic lupus erythematosus(SLE) and its relationship with the clinical stages of SLE. METHODS: The full blood culture and flow cytometry with fluorescence-labelled T lymphocytes were used to detect the levels of T-lymphocyte subsets and its intracellular factors IFN-γ and IL-4 in peripheral blood from SLP patients in active, inactive stages and normal healthy persons as controls, to compare the changes of Tc1,Tc2; Th1,Th2 levels and Tc/Th ratio in SLP patients in active and inactive stages, and to analyze their relationship with SLEDAI staging. RESULTS: Compared with healthy controls, the levels of CD4+/CD8+ in active SLEpatients were significantly lower, but the levels of Tc1,Th1 in active SLEpatients were higher than those in inactive SLEpatients and normal controls. The ratio of Tc1/Tc2 and Th1/Th2 in the active SLE was higher than that in inactive SLE and normal controls(P<0.05), but the difference between inactive SLEpatients and controls was not statistically significant (P>0.05). CONCLUSION: The Tc/Th imbalances and change of Tc and Th cells to Tc1 and Th1 cells play an important role in the pathogenesis of SLE.