Karin A Binnemars-Postma1, Hetty Wm Ten Hoopen1, Gert Storm1,2, Jai Prakash1. 1. Department of Biomaterials Science & Technology, Targeted Therapeutics Section, MIRA Institute for Biomedical Technology & Technical Medicine, University of Twente, Enschede, The Netherlands. 2. Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
Abstract
AIM: To investigate the interaction behavior of M1- and M2-type macrophages with nanoparticles of different sizes with/without the presence of serum. MATERIALS & METHODS: THP-1 human monocytes were differentiated into M1 and M2 macrophages, and the uptake of silica nanoparticle (50-1000 nm) was studied using flow cytometry and different microscopies. RESULTS: Without serum, higher uptake of all-sized nanoparticles was observed by M1 compared with M2. With serum, uptake of nanoparticles (200-1000 nm) was dramatically increased by M2. Furthermore, serum proteins adsorbed (corona) by nanoparticles were found to be the ligands for receptors expressed by M2, as revealed by SDS-PAGE and gene profiling analyses. CONCLUSION: The observed differential uptake by M1 and M2 macrophages will help understand the fate of nanoparticles in vivo.
AIM: To investigate the interaction behavior of M1- and M2-type macrophages with nanoparticles of different sizes with/without the presence of serum. MATERIALS & METHODS: THP-1 human monocytes were differentiated into M1 and M2 macrophages, and the uptake of silica nanoparticle (50-1000 nm) was studied using flow cytometry and different microscopies. RESULTS: Without serum, higher uptake of all-sized nanoparticles was observed by M1 compared with M2. With serum, uptake of nanoparticles (200-1000 nm) was dramatically increased by M2. Furthermore, serum proteins adsorbed (corona) by nanoparticles were found to be the ligands for receptors expressed by M2, as revealed by SDS-PAGE and gene profiling analyses. CONCLUSION: The observed differential uptake by M1 and M2 macrophages will help understand the fate of nanoparticles in vivo.
Authors: Glen M DeLoid; Yanli Wang; Klara Kapronezai; Laura Rubio Lorente; Roujie Zhang; Georgios Pyrgiotakis; Nagarjun V Konduru; Maria Ericsson; Jason C White; Roberto De La Torre-Roche; Hang Xiao; David Julian McClements; Philip Demokritou Journal: Part Fibre Toxicol Date: 2017-10-13 Impact factor: 9.400