Properties, in theory and practice, of novel gel filtration media for standard liquid chromatography.

The influence of experimental parameters on the separation result in gel filtration may readily be predicted from a few basic equations, as demonstrated with the aid of experimental observations on Sephacryl HR. Excellent agreement between the predicted resolution and that determined experimentally was found for parameters such as temperature, particle size, column length, flow velocity, sample volume and gel porosity. The theoretical prediction of an optimum sample volume for a constant processing rate was also experimentally verified. An exhaustive investigation of the physical, chemical and functional properties of Sephacryl HR was undertaken to facilitate the interpretation of experimental observations.