Ying Jin1, Huijin Chen, Xiaojian Xu, Yuntao Hu, Changguan Wang, Zhizhong Ma. 1. *Department of Ophthalmology, Peking University Third Hospital, Ministry of Education's Key Laboratory of Vision Loss and Restoration, Beijing, People's Republic of China; and †Department of Ophthalmology, Beijing Tsinghua Changgung Hospital, Beijing, People's Republic of China.
Abstract
PURPOSE: To determine the phases of traumatic proliferative vitreoretinopathy after open globe injury by assessing cellular components, extracellular matrix constituents of proliferative vitreoretinopathy membranes, and intraretinal changes over time. METHODS: Twenty-one epiretinal and/or subretinal membrane specimens were obtained from 21 patients with open globe injuries. The patients were divided into Groups A (≤28 days), B (29-120 days), and C (>120 days) according to the interval between injury and vitrectomy. The staining intensity and percentage of positive cells in membranes were compared among the groups, and proliferative indices for Ki-67 and proliferating cell nuclear antigen were assessed. Intraretinal changes were evaluated through histology and immunohistochemistry. Fundus photography was performed during vitrectomy. RESULTS: The proliferating cell nuclear antigen proliferative index was significantly higher in Group B (P = 0.002) than in Group A, and lower in Group C (P < 0.001) than in Group B. α-smooth muscle actin expression increased from day 29 to 120 after injury. Meanwhile, intraretinal gliosis and fibrosis developed. CONCLUSION: Active proliferation and contraction in proliferative vitreoretinopathy membranes continue until 120 days after injury, and are accompanied by the initiation of intraretinal gliosis and fibrosis. These findings provide further insight into the optimal timing of vitrectomy after trauma.
PURPOSE: To determine the phases of traumatic proliferative vitreoretinopathy after open globe injury by assessing cellular components, extracellular matrix constituents of proliferative vitreoretinopathy membranes, and intraretinal changes over time. METHODS: Twenty-one epiretinal and/or subretinal membrane specimens were obtained from 21 patients with open globe injuries. The patients were divided into Groups A (≤28 days), B (29-120 days), and C (>120 days) according to the interval between injury and vitrectomy. The staining intensity and percentage of positive cells in membranes were compared among the groups, and proliferative indices for Ki-67 and proliferating cell nuclear antigen were assessed. Intraretinal changes were evaluated through histology and immunohistochemistry. Fundus photography was performed during vitrectomy. RESULTS: The proliferating cell nuclear antigen proliferative index was significantly higher in Group B (P = 0.002) than in Group A, and lower in Group C (P < 0.001) than in Group B. α-smooth muscle actin expression increased from day 29 to 120 after injury. Meanwhile, intraretinal gliosis and fibrosis developed. CONCLUSION: Active proliferation and contraction in proliferative vitreoretinopathy membranes continue until 120 days after injury, and are accompanied by the initiation of intraretinal gliosis and fibrosis. These findings provide further insight into the optimal timing of vitrectomy after trauma.
Authors: Filippos Vingopoulos; Yvonne Wang; Seanna Grob; Chloe Yang Ling Li; Dean Eliott; Leo A Kim; Demetrios G Vavvas; John B Miller Journal: J Vitreoretin Dis Date: 2020-11-19
Authors: Bhav Harshad Parikh; Zengping Liu; Paul Blakeley; Qianyu Lin; Malay Singh; Jun Yi Ong; Kim Han Ho; Joel Weijia Lai; Hanumakumar Bogireddi; Kim Chi Tran; Jason Y C Lim; Kun Xue; Abdurrahmaan Al-Mubaarak; Binxia Yang; Sowmiya R; Kakkad Regha; Daniel Soo Lin Wong; Queenie Shu Woon Tan; Zhongxing Zhang; Anand D Jeyasekharan; Veluchamy Amutha Barathi; Weimiao Yu; Kang Hao Cheong; Timothy A Blenkinsop; Walter Hunziker; Gopal Lingam; Xian Jun Loh; Xinyi Su Journal: Nat Commun Date: 2022-05-19 Impact factor: 17.694