Literature DB >> 2777945

High-performance liquid chromatography of proteins on N-methylpyridinium polymer columns.

A Sugii1, K Harada, K Nishimura, R Hanaoka, S Masuda.   

Abstract

Two types of 4-methylpyridinium polymers (4VP-DVB-Me and 4VP-EG-Me, cross-linked with divinylbenzene and ethylene glycol dimethacrylate, respectively) were employed for the analysis of proteins in ion-exchange high-performance liquid chromatography. These polymers had different physical properties in the dry state, but showed similar retentions in size-exclusion chromatography using carbohydrate standards. Generally, the 4VP-EG-Me column was superior to the 4VP-DVB-Me column with regard to separation and recovery of proteins.

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Year:  1989        PMID: 2777945     DOI: 10.1016/s0021-9673(00)94134-7

Source DB:  PubMed          Journal:  J Chromatogr


  2 in total

1.  In vitro and in vivo properties of recombinant human serum albumin from Pichia pastoris purified by a method of short processing time.

Authors:  H Watanabe; K Yamasaki; U Kragh-Hansen; S Tanase; K Harada; A Suenaga; M Otagiri
Journal:  Pharm Res       Date:  2001-12       Impact factor: 4.200

2.  Kinetic analysis of covalent binding between N-acetyl-L-cysteine and albumin through the formation of mixed disulfides in human and rat serum in vitro.

Authors:  Daisuke Harada; Shinsuku Naito; Masaki Otagiri
Journal:  Pharm Res       Date:  2002-11       Impact factor: 4.200

  2 in total

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