Hai-Jing Yu1, Hua Deng2, Jian Ma2, Shu-Jun Huang2, Jian-Min Yang1, Yan-Fen Huang3, Xiao-Ping Mu3, Liang Zhang4, Qi Wang5. 1. Breast Disease Center, Guangdong Women and Children Hospital, Guangzhou 511400, PR China. 2. Translational Medicine Center, Guangdong Women and Children Hospital, Guangzhou, PR China. 3. Department of Clinical Laboratory, Guangdong Women and Children Hospital, Guangzhou, PR China. 4. Translational Medicine Center, Guangdong Women and Children Hospital, Guangzhou, PR China; Maternal and Children Metabolic-Genetic Key Laboratory, Guangdong Women and Children Hospital, Guangzhou, PR China. 5. Breast Disease Center, Guangdong Women and Children Hospital, Guangzhou 511400, PR China; Maternal and Children Metabolic-Genetic Key Laboratory, Guangdong Women and Children Hospital, Guangzhou, PR China. Electronic address: wangqigz@hotmail.com.
Abstract
BACKGROUND: Granulomatous mastitis (GM) is a chronic inflammatory breast lesion. Its etiology remains incompletely defined. Although mounting evidence suggests the involvement of Corynebacterium in GM, there has been no systematic study of GM bacteriology using -omics technology. METHODS: The bacterial diversity and relative abundances in breast abscesses from 19 women with GM were investigated using 16S rDNA metagenomic sequencing and Sanger sequencing. A quantitative PCR (qPCR) assay was also developed to identify Corynebacterium kroppenstedtii. RESULTS: A bioinformatic analysis revealed that Corynebacterium was present in the 19 GM patients, with abundances ranging from 1.1% to 58.9%. Of note, Corynebacterium was the most abundant taxon in seven patients (more than a third of the subjects). The predominance of Corynebacterium kroppenstedtii infection (11 of 19 patients, 57.9%) was confirmed with Sanger sequencing and the qPCR assay. CONCLUSIONS: This study profiled the microbiota of patients with GM and indicated an important role for Corynebacterium, and in particular C. kroppenstedtii, in the pathogenesis of this disease.
BACKGROUND:Granulomatous mastitis (GM) is a chronic inflammatory breast lesion. Its etiology remains incompletely defined. Although mounting evidence suggests the involvement of Corynebacterium in GM, there has been no systematic study of GM bacteriology using -omics technology. METHODS: The bacterial diversity and relative abundances in breast abscesses from 19 women with GM were investigated using 16S rDNA metagenomic sequencing and Sanger sequencing. A quantitative PCR (qPCR) assay was also developed to identify Corynebacterium kroppenstedtii. RESULTS: A bioinformatic analysis revealed that Corynebacterium was present in the 19 GM patients, with abundances ranging from 1.1% to 58.9%. Of note, Corynebacterium was the most abundant taxon in seven patients (more than a third of the subjects). The predominance of Corynebacterium kroppenstedtii infection (11 of 19 patients, 57.9%) was confirmed with Sanger sequencing and the qPCR assay. CONCLUSIONS: This study profiled the microbiota of patients with GM and indicated an important role for Corynebacterium, and in particular C. kroppenstedtii, in the pathogenesis of this disease.
Authors: Sally C Y Wong; Rosana W S Poon; Jonathan H K Chen; Herman Tse; Janice Y C Lo; Tak-Keung Ng; Jonathan C K Au; Cindy W S Tse; Ingrid Y Y Cheung; Man-Ting Yuk; Wei-Kwang Luk; Kwok-Yung Yuen Journal: Open Forum Infect Dis Date: 2017-05-06 Impact factor: 3.835