Olga Kostianets1, Maksym Shyyan1, Stepan Vasil'ovich Antoniuk2, Valeriy Filonenko1, Ramziya Kiyamova1,3. 1. a Department of Cell Signaling , Institute of Molecular Biology and Genetics, NAS of Ukraine , Kyiv , Ukraine. 2. b Dnipropetrovsk Regional Center of Pathology , Kyiv , Ukraine. 3. c Kazan Volga Region Federal University, Institute of Fundamental Medicine and Biology , Kazan , Russian Federation.
Abstract
CONTENT: Identification of panel of SEREX-defined antigens for breast cancer autoantibodies profile detection. OBJECTIVE: To create panel of antigens that can differentiate breast cancer patients and healthy individuals. METHODS: SEREX (serological analysis of cDNA expression libraries) method, ELISA (enzyme-linked immunosorbent assay), qPCR (quantitative polymerase chain reaction). RESULTS: In large-scale screening of 16 SEREX-antigens by sera of breast cancer patients and healthy donors, a combination of six antigens (RAD50, PARD3, SPP1, SAP30BP, NY-BR-62 and NY-CO-58) was identified, which can differentiate breast cancer patients and healthy donors with 70% sensitivity and 91% specificity. Elevated mRNA expression of SPP1 gene was revealed in breast tumors (2-7-fold) that correlated with SPP1 antigen immunoreactivity in autologous patients' sera. CONCLUSIONS: The new panel of six SEREX-antigens was proposed, which enables creation of serological assay for breast cancer diagnostics and/or prognosis.
CONTENT: Identification of panel of SEREX-defined antigens for breast cancer autoantibodies profile detection. OBJECTIVE: To create panel of antigens that can differentiate breast cancerpatients and healthy individuals. METHODS: SEREX (serological analysis of cDNA expression libraries) method, ELISA (enzyme-linked immunosorbent assay), qPCR (quantitative polymerase chain reaction). RESULTS: In large-scale screening of 16 SEREX-antigens by sera of breast cancerpatients and healthy donors, a combination of six antigens (RAD50, PARD3, SPP1, SAP30BP, NY-BR-62 and NY-CO-58) was identified, which can differentiate breast cancerpatients and healthy donors with 70% sensitivity and 91% specificity. Elevated mRNA expression of SPP1 gene was revealed in breast tumors (2-7-fold) that correlated with SPP1 antigen immunoreactivity in autologous patients' sera. CONCLUSIONS: The new panel of six SEREX-antigens was proposed, which enables creation of serological assay for breast cancer diagnostics and/or prognosis.
Entities:
Keywords:
Tumor-associated antigens; antigenic panel; autoantibody; breast cancer
Authors: Sasha E Stanton; Ekram Gad; Erik Ramos; Lauren Corulli; James Annis; Jennifer Childs; Hiroyuki Katayama; Samir Hanash; Jeffrey Marks; Mary L Disis Journal: NPJ Breast Cancer Date: 2021-05-11