Hena Naqvi1, Mohammad Kaleem Ahmad2, Syed Rizwan Hussain1, Shalini Gupta3, Mohammad Waseem1, Abbas Ali Mahdi1. 1. Molecular Cell Biology Lab, Department of Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh, 226 003, India. 2. Molecular Cell Biology Lab, Department of Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh, 226 003, India. kaleembaksh@gmail.com. 3. Department of Oral Pathology & Microbiology, King George's Medical University, Lucknow, Uttar Pradesh, 226 003, India.
Abstract
BACKGROUND: Oral squamous cell carcinoma (OSCC) occurrence appears to be the number one among all cancers in India. Folate is a methyl donor during DNA methylation, as it provides substrate for methylenetetrahydrofolate reductase (MTHFR) to convert 5,10-MTHF to 5-MTHF and subsequently metabolizes it to methionine. The purpose of this study was to identify MTHFR C677T gene polymorphism in patients with OSCC. MATERIALS AND METHODS: A total of 350 OSCC cases and 350 healthy controls participated in this study. MTHFR C677T single-nucleotide polymorphism was evaluated by PCR-RFLP. RESULTS: In the present study, MTHFR gene 677CC, CT, and TT genotype frequencies of the total OSCC cases were 74.8; 19.4 and 5.71; and 88.5, 9.42, and 2.0 % in controls. The average frequency of the MTHFR 677T allele was 15.4 % in OSCC cases compared to 6.71 % in the controls. The CT genotype occurrence prevailed more in patients than controls in contrast to TT genotype, although both the genotypes were statistically significant for OSCC. Moreover, we found that T allele was significant in cases of smoking and tobacco chewing. CONCLUSIONS: In this study, we found that the homozygous mutant T allele appeared to have significantly higher risk of OSCC especially in late stages and therefore supporting in OSCC susceptibility and its progression.
BACKGROUND: Oral squamous cell carcinoma (OSCC) occurrence appears to be the number one among all cancers in India. Folate is a methyl donor during DNA methylation, as it provides substrate for methylenetetrahydrofolate reductase (MTHFR) to convert 5,10-MTHF to 5-MTHF and subsequently metabolizes it to methionine. The purpose of this study was to identify MTHFRC677T gene polymorphism in patients with OSCC. MATERIALS AND METHODS: A total of 350 OSCC cases and 350 healthy controls participated in this study. MTHFRC677T single-nucleotide polymorphism was evaluated by PCR-RFLP. RESULTS: In the present study, MTHFR gene 677CC, CT, and TT genotype frequencies of the total OSCC cases were 74.8; 19.4 and 5.71; and 88.5, 9.42, and 2.0 % in controls. The average frequency of the MTHFR 677T allele was 15.4 % in OSCC cases compared to 6.71 % in the controls. The CT genotype occurrence prevailed more in patients than controls in contrast to TT genotype, although both the genotypes were statistically significant for OSCC. Moreover, we found that T allele was significant in cases of smoking and tobacco chewing. CONCLUSIONS: In this study, we found that the homozygous mutant T allele appeared to have significantly higher risk of OSCC especially in late stages and therefore supporting in OSCC susceptibility and its progression.
Authors: E Vairaktaris; C Yapijakis; P Kessler; A Vylliotis; J Ries; J Wiltfang; S Vassiliou; S Derka; F W Neukam Journal: J Cancer Res Clin Oncol Date: 2005-12-20 Impact factor: 4.553
Authors: Ana S Neumann; Heather J Lyons; Hongbing Shen; Zhensheng Liu; Qiuling Shi; Erich M Sturgis; Sanjay Shete; Margaret R Spitz; Adel El-Naggar; Waun Ki Hong; Qingyi Wei Journal: Int J Cancer Date: 2005-05-20 Impact factor: 7.396
Authors: Stephanie J Weinstein; Gloria Gridley; Lea C Harty; Scott R Diehl; Linda M Brown; Deborah M Winn; Eleuterio Bravo-Otero; Richard B Hayes Journal: J Nutr Date: 2002-04 Impact factor: 4.798