Literature DB >> 27774499

Genetic Transformation of Candida glabrata by Electroporation.

Fabian Istel1, Tobias Schwarzmüller1, Michael Tscherner1, Karl Kuchler1.   

Abstract

Here, we report a method for the transformation by electroporation of the human fungal pathogen Candida glabrata (C. glabrata). The protocol can be used for transformations in single well or in 96-well microtiter plates. It has been extensively used to generate a genome-scale gene deletion library using the C. glabrata background recipient strain ATCC2001 (Schwarzmüller et al., 2014).

Entities:  

Year:  2015        PMID： 27774499      PMCID： PMC5074361          DOI： 10.21769/BioProtoc.1528

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  2 in total

1.  The SAT1 flipper, an optimized tool for gene disruption in Candida albicans.

Authors:  Oliver Reuss; Ashild Vik; Roberto Kolter; Joachim Morschhäuser
Journal:  Gene       Date:  2004-10-27       Impact factor: 3.688

2.  Systematic phenotyping of a large-scale Candida glabrata deletion collection reveals novel antifungal tolerance genes.

Authors:  Tobias Schwarzmüller; Biao Ma; Ekkehard Hiller; Fabian Istel; Michael Tscherner; Sascha Brunke; Lauren Ames; Arnaud Firon; Brian Green; Vitor Cabral; Marina Marcet-Houben; Ilse D Jacobsen; Jessica Quintin; Katja Seider; Ingrid Frohner; Walter Glaser; Helmut Jungwirth; Sophie Bachellier-Bassi; Murielle Chauvel; Ute Zeidler; Dominique Ferrandon; Toni Gabaldón; Bernhard Hube; Christophe d'Enfert; Steffen Rupp; Brendan Cormack; Ken Haynes; Karl Kuchler
Journal:  PLoS Pathog       Date:  2014-06-19       Impact factor: 6.823
  2 in total
  4 in total

1.  A common vesicle proteome drives fungal biofilm development.

Authors:  Robert Zarnowski; Hiram Sanchez; Anna Jaromin; Urszula J Zarnowska; Jeniel E Nett; Aaron P Mitchell; David Andes
Journal:  Proc Natl Acad Sci U S A       Date:  2022-09-12       Impact factor: 12.779

2.  Characterization of the substrate binding site of an iron detoxifying membrane transporter from Plasmodium falciparum.

Authors:  Pragya Sharma; Veronika Tóth; Edel M Hyland; Christopher J Law
Journal:  Malar J       Date:  2021-06-30       Impact factor: 2.979

3.  Conservation and Divergence in the Candida Species Biofilm Matrix Mannan-Glucan Complex Structure, Function, and Genetic Control.

Authors:  Eddie Dominguez; Robert Zarnowski; Hiram Sanchez; Antonio S Covelli; William M Westler; Parastoo Azadi; Jeniel Nett; Aaron P Mitchell; David R Andes
Journal:  mBio       Date:  2018-04-03       Impact factor: 7.867

4.  Novel FKS1 and FKS2 modifications in a high-level echinocandin resistant clinical isolate of Candida glabrata.

Authors:  Xin Hou; Kelley R Healey; Erika Shor; Milena Kordalewska; Cristina Jiménez Ortigosa; Padmaja Paderu; Meng Xiao; He Wang; Ying Zhao; Li-Yan Lin; Yan-Hai Zhang; Yong-Zhe Li; Ying-Chun Xu; David S Perlin; Yanan Zhao
Journal:  Emerg Microbes Infect       Date:  2019       Impact factor: 7.163
  4 in total

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