Literature DB >> 2777384

Immunoelectron microscopy of lipopolysaccharide in Chlamydia trachomatis.

S Birkelund1, A G Lundemose, G Christiansen.   

Abstract

Monoclonal antibodies (MAb) specific for Chlamydia trachomatis lipopolysaccharide (LPS) and major outer membrane protein (MOMP) were used for immunoelectron microscopy analysis. MAb specific for MOMP showed strong reaction with the chlamydial surface, whereas MAb specific for LPS showed strong association of gold particles with the periphery of the chlamydial body. After fixation of the chlamydia cells, the reactivity was, however, similar to the anti-MOMP reactivity. Enzyme-linked immunosorbent assay showed that MAb specific for LPS could remove LPS from the chlamydial membrane.

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Year:  1989        PMID： 2777384      PMCID： PMC260801          DOI： 10.1128/iai.57.10.3250-3253.1989

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  20 in total

1.  Chemical cross-linking of Chlamydia trachomatis.

Authors:  S Birkelund; A G Lundemose; G Christiansen
Journal:  Infect Immun       Date:  1988-03       Impact factor: 3.441

2.  Non-specific binding of protein-stabilized gold sols as a source of error in immunocytochemistry.

Authors:  O Behnke; T Ammitzbøll; H Jessen; M Klokker; K Nilausen; J Tranum-Jensen; L Olsson
Journal:  Eur J Cell Biol       Date:  1986-08       Impact factor: 4.492

3.  Ultrastructural localization of specific gonococcal macromolecules with antibody-gold sphere immunological probes.

Authors:  E N Robinson; Z A McGee; J Kaplan; M E Hammond; J K Larson; T M Buchanan; G K Schoolnik
Journal:  Infect Immun       Date:  1984-11       Impact factor: 3.441

4.  Expression of the chlamydial genus-specific lipopolysaccharide epitope in Escherichia coli.

Authors:  F E Nano; H D Caldwell
Journal:  Science       Date:  1985-05-10       Impact factor: 47.728

5.  Probing the surface of Neisseria gonorrhoeae: simultaneous localization of protein I and H.8 antigens.

Authors:  E N Robinson; Z A McGee; T M Buchanan; M S Blake; P J Hitchcock
Journal:  Infect Immun       Date:  1987-05       Impact factor: 3.441

6.  Tear and serum antibody response to Chlamydia trachomatis antigens during acute chlamydial conjunctivitis in monkeys as determined by immunoblotting.

Authors:  H D Caldwell; S Stewart; S Johnson; H Taylor
Journal:  Infect Immun       Date:  1987-01       Impact factor: 3.441

7.  Attempted oral immunization with chlamydial lipopolysaccharide subunit vaccine.

Authors:  H R Taylor; R A Prendergast
Journal:  Invest Ophthalmol Vis Sci       Date:  1987-10       Impact factor: 4.799

8.  Protective monoclonal antibodies recognize epitopes located on the major outer membrane protein of Chlamydia trachomatis.

Authors:  Y X Zhang; S Stewart; T Joseph; H R Taylor; H D Caldwell
Journal:  J Immunol       Date:  1987-01-15       Impact factor: 5.422

9.  C3 binds preferentially to long-chain lipopolysaccharide during alternative pathway activation by Salmonella montevideo.

Authors:  K A Joiner; N Grossman; M Schmetz; L Leive
Journal:  J Immunol       Date:  1986-01       Impact factor: 5.422

10.  Chemical and serological investigations on the genus-specific lipopolysaccharide epitope of Chlamydia.

Authors:  H Brade; L Brade; F E Nano
Journal:  Proc Natl Acad Sci U S A       Date:  1987-04       Impact factor: 11.205
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  7 in total

1.  Staining of surface antigens of Chlamydia trachomatis L2 in tissue culture.

Authors:  M Baumann; L Brade; E Fasske; H Brade
Journal:  Infect Immun       Date:  1992-10       Impact factor: 3.441

2.  Topological analysis of Chlamydia trachomatis L2 outer membrane protein 2.

Authors:  P Mygind; G Christiansen; S Birkelund
Journal:  J Bacteriol       Date:  1998-11       Impact factor: 3.490

3.  Induction of alpha/beta interferon and dependent nitric oxide synthesis during Chlamydia trachomatis infection of McCoy cells in the absence of exogenous cytokine.

Authors:  A Devitt; P A Lund; A G Morris; J H Pearce
Journal:  Infect Immun       Date:  1996-10       Impact factor: 3.441

4.  Chlamydia and sudden infant death syndrome. A study of 166 SIDS and 30 control cases.

Authors:  J B Lundemose; A G Lundemose; M Gregersen; K Helweg-Larsen; J Simonsen
Journal:  Int J Legal Med       Date:  1990-12       Impact factor: 2.686

Review 5.  Diagnosis and assessment of trachoma.

Authors:  Anthony W Solomon; Rosanna W Peeling; Allen Foster; David C W Mabey
Journal:  Clin Microbiol Rev       Date:  2004-10       Impact factor: 26.132

6.  Induction of interferon-stimulated genes by Chlamydia pneumoniae in fibroblasts is mediated by intracellular nucleotide-sensing receptors.

Authors:  Srikanth Chiliveru; Svend Birkelund; Søren R Paludan
Journal:  PLoS One       Date:  2010-04-06       Impact factor: 3.240

7.  Establishment of a particle-counting method for purified elementary bodies of chlamydiae and evaluation of sensitivities of the IDEIA Chlamydia kit and DNA probe by using the purified elementary bodies.

Authors:  N Miyashita; A Matsumoto
Journal:  J Clin Microbiol       Date:  1992-11       Impact factor: 5.948
  7 in total

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