| Literature DB >> 27771505 |
Yanzhu Zhu1, Feibo Xu2, Xijun Yan3, Liguang Miao3, Haitao Li3, Chongwei Hu4, Zhongying Wang5, Shizhen Lian3, Zhuo Feng3, Yanfei Li6.
Abstract
Aluminum (Al) exposure impairs bone formation, and bone formation is mediated by the osteoblasts. But effects of Al on the osteoblasts function remain elusive. The osteoblasts were exposed to 0, 0.0252, 0.126, 0.252mg/mL AlCl3·6H2O for 24h. The osteoblasts viability, TGF-β1, BMP-2, IGF-I and Cbfα1 mRNA expressions, and GSH-Px and SOD activities, ROS concentration were determined. The osteoblasts ultrastructural features were also observed. The results showed that AlCl3 suppressed the osteoblasts viability, TGF-β1, BMP-2, IGF-I and Cbfα1 mRNA expressions, GSH-Px and SOD activities, and elevated ROS concentration compared with the CG. The ultrastructural features of osteoblasts in the HG showed mitochondrial swelling, foam-like structure, uneven distribution of chromatin, incomplete cell membrane and cytoplasm spillover compared with the CG. It indicates that AlCl3 inhibits osteoblasts viability, growth regulation factors mRNA expressions, anti-oxidative function, and damaged the osteoblasts histology structure, impairing the osteoblasts function. Copyright ÂEntities:
Keywords: Aluminum; Anti-oxidative function; Growth regulation factors mRNA expressions; Osteoblasts viability; Ultrastructural features
Mesh：
Substances：
Year: 2016 PMID： 27771505 DOI： 10.1016/j.etap.2016.10.009
Source DB: PubMed Journal: Environ Toxicol Pharmacol ISSN： 1382-6689 Impact factor: 4.860