Ria Arnold1, Mihai Moldovan2, Mette Romer Rosberg2, Arun V Krishnan3, Renee Morris4, Christian Krarup2. 1. School of Medical Sciences, University of New South Wales, Sydney, Australia. Electronic address: ria.arnold@unsw.edu.au. 2. Department of Clinical Neurophysiology, Rigshospitalet and the Institute of Neuroscience and Pharmacology, University of Copenhagen, Copenhagen, Denmark. 3. Prince of Wales Clinical School, University of New South Wales, Sydney, Australia. 4. School of Medical Sciences, University of New South Wales, Sydney, Australia.
Abstract
BACKGROUND: Nerve excitability testing by threshold-tracking is the only available method to study axonal ion channel function and membrane potential in the clinical setting. The measures are, however, indirect and the interpretation of neuropathic changes remains challenging. The same multiple measures of axonal excitability were adapted to further explore the pathophysiological changes in rodent disease models under pharmacologic and genetic manipulations. These studies are typically limited to the investigation of the "long nerves" such as the tail or the tibial nerves. NEW METHOD: We introduce a novel setup to explore the ulnar nerve excitability in rodents. We provide normative ulnar data in 11 adult female Long Evans rats under anaesthesia by comparison with tibial and caudal nerves. Additionally, these measures were repeated weekly on 3 occasions to determine the repeatability of these tests. RESULTS: Nerve excitability assessment of ulnar nerve proved to be a longitudinally repeatable measure of axonal function mature in rats, as were measures in tibial and caudal nerves. Comparison with existing method: Ulnar nerve motor excitability measures were different from the caudal and tibial excitability measures. Most notably, ulnar nerve showed the largest threshold changes during both depolarizing and hyperpolarizing threshold electrotonus. CONCLUSIONS: Ulnar nerves demonstrate a distinct nerve excitability profile than the caudal and tibial nerves which could have functional and pathological implications. Copyright Â
BACKGROUND: Nerve excitability testing by threshold-tracking is the only available method to study axonal ion channel function and membrane potential in the clinical setting. The measures are, however, indirect and the interpretation of neuropathic changes remains challenging. The same multiple measures of axonal excitability were adapted to further explore the pathophysiological changes in rodent disease models under pharmacologic and genetic manipulations. These studies are typically limited to the investigation of the "long nerves" such as the tail or the tibial nerves. NEW METHOD: We introduce a novel setup to explore the ulnar nerve excitability in rodents. We provide normative ulnar data in 11 adult female Long Evans rats under anaesthesia by comparison with tibial and caudal nerves. Additionally, these measures were repeated weekly on 3 occasions to determine the repeatability of these tests. RESULTS: Nerve excitability assessment of ulnar nerve proved to be a longitudinally repeatable measure of axonal function mature in rats, as were measures in tibial and caudal nerves. Comparison with existing method: Ulnar nerve motor excitability measures were different from the caudal and tibial excitability measures. Most notably, ulnar nerve showed the largest threshold changes during both depolarizing and hyperpolarizing threshold electrotonus. CONCLUSIONS: Ulnar nerves demonstrate a distinct nerve excitability profile than the caudal and tibial nerves which could have functional and pathological implications. Copyright Â
Authors: Brandon M Wild; Renée Morris; Mihai Moldovan; Christian Krarup; Arun V Krishnan; Ria Arnold Journal: J Vis Exp Date: 2018-02-06 Impact factor: 1.355
Authors: Markus E Harrigan; Angela R Filous; Andrew P Tosolini; Renee Morris; Jan M Schwab; W David Arnold Journal: Sci Rep Date: 2019-11-13 Impact factor: 4.379