Amalia Pérez-Jiménez1, Eva E Rufino-Palomares2, Nieves Fernández-Gallego3, M Carmen Ortuño-Costela3, Fernando J Reyes-Zurita3, Juan Peragón4, Leticia García-Salguero3, Khalida Mokhtari5, Pedro P Medina6, José A Lupiáñez7. 1. Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, Spain; Department of i+D+I, Biomaslinic S.L., Polígono Industrial de Escúzar, 18130 Granada, Spain. Electronic address: calaya@ugr.es. 2. Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, Spain; Pfizer Pharmaceutical-University of Granada-Government of Andalusian, Centre of Genomic and Oncologic Investigation (GENyO), Technological Park of Health Sciences, 18016 Granada, Spain. Electronic address: evaevae@ugr.es. 3. Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, Spain. 4. Department of Experimental Biology, Biochemistry Section, Faculty of Experimental Biology, University of Jaén, 23071 Jaén, Spain. 5. Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, Spain; Department of Biology, Faculty of Sciences, Mohammed I University, BP 717 60000 Oujda, Morocco. 6. Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, Spain; Pfizer Pharmaceutical-University of Granada-Government of Andalusian, Centre of Genomic and Oncologic Investigation (GENyO), Technological Park of Health Sciences, 18016 Granada, Spain. 7. Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, 18071 Granada, Spain. Electronic address: jlcara@ugr.es.
Abstract
BACKGROUND: Metabolic syndrome is a set of pathologies among which stand out the obesity, which is related to the lipid droplet accumulation and changes to cellular morphology regulated by several molecules and transcription factors. Maslinic acid (MA) is a natural product with demonstrated pharmacological functions including anti-inflammation, anti-tumor and anti-oxidation, among others. PURPOSE: Here we report the effects of MA on the adipogenesis process in 3T3-L1 cells. METHODS: Cell viability, glucose uptake, cytoplasmic triglyceride droplets, triglycerides quantification, gene transcription factors such as peroxisome proliferator-activated receptor γ (PPARγ) and adipocyte fatty acid-binding protein (aP2) and intracellular Ca2+ levels were determined in pre-adipocytes and adipocytes of 3T3-L1 cells. RESULTS: MA increased glucose uptake. MA also decreased lipid droplets and triglyceride levels, which is in concordance with the down-regulation of PPARγ and aP2. Finally, MA increased the intracellular Ca2+ concentration, which could also be involved in the demonstrated antiadipogenic effect of this triterpene. CONCLUSION: MA has been demonstrated as potential antiadipogenic compound in 3T3-L1 cells.
BACKGROUND:Metabolic syndrome is a set of pathologies among which stand out the obesity, which is related to the lipid droplet accumulation and changes to cellular morphology regulated by several molecules and transcription factors. Maslinic acid (MA) is a natural product with demonstrated pharmacological functions including anti-inflammation, anti-tumor and anti-oxidation, among others. PURPOSE: Here we report the effects of MA on the adipogenesis process in 3T3-L1 cells. METHODS: Cell viability, glucose uptake, cytoplasmic triglyceride droplets, triglycerides quantification, gene transcription factors such as peroxisome proliferator-activated receptor γ (PPARγ) and adipocyte fatty acid-binding protein (aP2) and intracellular Ca2+ levels were determined in pre-adipocytes and adipocytes of 3T3-L1 cells. RESULTS: MA increased glucose uptake. MA also decreased lipid droplets and triglyceride levels, which is in concordance with the down-regulation of PPARγ and aP2. Finally, MA increased the intracellular Ca2+ concentration, which could also be involved in the demonstrated antiadipogenic effect of this triterpene. CONCLUSION: MA has been demonstrated as potential antiadipogenic compound in 3T3-L1 cells.
Authors: Alberto M Parra-Perez; Amalia Pérez-Jiménez; Isabel Gris-Cárdenas; Gloria C Bonel-Pérez; Luis M Carrasco-Díaz; Khalida Mokhtari; Leticia García-Salguero; José A Lupiáñez; Eva E Rufino-Palomares Journal: Int J Mol Sci Date: 2022-06-24 Impact factor: 6.208
Authors: Carmen Maria Claro-Cala; Jose Carlos Quintela; Marta Pérez-Montero; Javier Miñano; María Alvarez de Sotomayor; María Dolores Herrera; And Rosalía Rodríguez-Rodríguez Journal: Nutrients Date: 2020-01-26 Impact factor: 5.717
Authors: Gloria C Bonel-Pérez; Amalia Pérez-Jiménez; Isabel Gris-Cárdenas; Alberto M Parra-Pérez; José Antonio Lupiáñez; Fernando J Reyes-Zurita; Eva Siles; René Csuk; Juan Peragón; Eva E Rufino-Palomares Journal: Molecules Date: 2020-09-16 Impact factor: 4.411
Authors: Juan Luis Peñas-Fuentes; Eva Siles; Eva E Rufino-Palomares; Amalia Pérez-Jiménez; Fernando J Reyes-Zurita; José A Lupiáñez; Carlos Fuentes-Almagro; Juan Peragón-Sánchez Journal: Antioxidants (Basel) Date: 2021-12-29